Study of Polymorphism in a-Lactalbumin and b-Lactoglobulin Genes in Marathwadi Buffalo
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Date
2016
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Publisher
MAFSU, Nagpur
Abstract
The present research work was conducted to study the polymorphism
of -Lactalbumin and -Lactoglobulin genes (whey protein genes) in
Marathwadi buffaloes using PCR-RFLP technique. The experiment material
was comprised of (n=30) blood samples and (n=9) milk samples of
Marathwadi buffaloes, collected from the Marathwadi Buffalo Farm, College of
Veterinary and Animal Sciences, Udgir during June-August, 2016.
The research work was conducted at Molecular Genetics Laboratory
of Department of Animal Genetics and Breeding, Post Graduate Institute of
Veterinary and Animal Sciences, Akola. DNA was isolated from blood using
standard Phenol-Chloroform extraction method followed by checking of its quality and quantity by Spectrophotometer and subsequently by
electrophoresis method.
A 309 bp fragment of -Lactalbumin gene amplified using forward (5’
TTG GTT TTA CTG GCC TCT CTT GTC ATC 3’) and reverse (5’ TGA ATT
ATG GGA CAA AGC AAA ATA GCA G 3’) primer with MspI restriction
enzyme yielded only one type of restriction pattern (220 bp and 89 bp) in all
30 Marathwadi buffaloes and accordingly monomorphic genotype was
identified as BB.
A 247 bp fragment of -Lactoglobulin (LGB) gene amplified using
forward (5’ TGT GCT GGA CAC CGA CTA CAA AAA 3’) and reverse (5’ GCT
CCC GGT ATA TGA CCA CCC TCT 3’) primer with HaeIII restriction enzyme
yielded only one type of restriction pattern (148, 99 and 74 bp) in all 30
Marathwadi buffaloes and accordingly monomorphic genotype was identified
as AB.
The estimates of protein, fat and lactose contents were recorded
thrice at monthly interval from 9 unrelated lactating buffaloes belonging to
Marathwadi Buffalo Farm, College of Veterinary and Animal Sciences, Udgir
using Lactoscan (Milk analyzer) during June to August 2016. The mean
values for protein, fat and Lactose content in Marathwadi buffalo ranged from
3.8 percent to 4.3 percent, 3.9 percent to 5.8 percent and 3.7 percent to 4.2
percent respectively. The total yields for protein, fat and Lactose content in
Marathwadi buffalo estimated by Test Interval Method ranged from 19.66 kg
to 30.52 kg, 21.99 kg to 31.11 kg and 18.68 kg to 28.65 kg, respectively.
The monomorphic pattern revealed by screening of -Lactalbumin
gene (BB) and -Lactoglobulin gene (AB) in small population of Marathwadi
buffaloes pointed out the need of genotyping whole or larger population of
‘true to breed type’ Marathwadi buffaloes from its breeding tract so as to
identify possible genotypes of whey protein genes. The monomorphic
genotype AB observed for -LG gene in Marathwadi buffaloes is suggestive
of presence of AA and BB genotypes in population of Marathwadi buffaloes.
Screening larger population of Marathwadi buffaloes for finding
polymorphism of candidate milk genes like whey protein genes is prerequisite
to explore their association with economically important milk production traits
will provide a robust platform for marker assisted selection.