EFFICACY OF ARGYROPHILIC NUCLEOLAR ORGANIZER REGION COUNT TEST FOR in vivo BIOMATERIAL EVALUATION
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Date
2001
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
The efficacy of Argyrophilic Nucleolar Organizer
Region (AgNOR) Count Test in the evaluation of biomaterial
was assessed in this study utilising a reactive (medium
chromic catgut) and a non-reactive (polypropylene)
material.
The materials were implanted into rat gluteus muscle
for a duration of seven and 14 days and sham surgery done
on a separate group of animals served as the control.
The tissue responses such as inflammation, muscle
degeneration and peri-implant scar thickness around the
reactive and non-reactive implant and the controls at seven
and 14 days were studied using H&E and special stains for
collagen. Besides this the estimation of collagen of the
reactive and non-reactive material implanted tissue was
studied. The observations made in this were compared with
the fibroblast proliferation response at the respective
durations as assessed by the AgNOR count estimation.
On histopathological evaluation, the reactive material
showed an initial acute inflammatory response with mild
fibrosis and collagen deposition which subsequently settled
into a chronic form with severe fibrosis and more collagen
deposition at the end of 14 days. Non-reactive material
elicited an acute inflammation initially (seven days post
implantation) which settled into a mild chronic response at
14 days post implantation. Mild fibrosis and less collagen
deposition were noticed in the peri-implant area at both
the duration. Observations on fibroblast proliferation as
assessed by AgNOR count test well correlated to the above
findings. The AgNOR counts observed with reactive material
at seven days and 14 days were 1.97 ± 0.03 and 2.23 ± 0.02
respectively. The counts for non-reactive material were
1.81 ± 0.01 and 1.67 ± 0.02 at seven and 14 days post
implantation respectively.
The collagen content of the reactive and non-reactive
implanted tissue did not reveal any statistically
significant difference as compared with the respective
control. From this investigation it was proved that AgNOR
count test could be reliably applied for biomaterial
evaluation even at seven days duration and it formed a
valuable adjunct to the routine method employed in the in
vivo evaluation of biomaterials.
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