Comparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicks

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dc.contributor.advisorSarvaiya, J. G.
dc.contributor.authorKALIA, ANIL KUMAR
dc.date.accessioned2018-06-21T09:25:25Z
dc.date.available2018-06-21T09:25:25Z
dc.date.issued2004
dc.description.abstractThe present work was planned to study induction of CYP3A and CYP2HI genes by Reverse Transcriptase polymerase chain reaction (RT-PCR) and Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Legiiorn chicles. Total RNA was extracted from the liver tissue samples using Tri Reagent based method. The quantity of extracted RNA was assessed spectrophotometrically at 260/280nm, and it ranged from 1.7 to 2.0 OD suggesting good quantity of RNA extraction. The quality of extracted RNA was checked by 1% formaldehyde agarose gel electrophoresis and it showed bands at 28s, 18s and 5s rRNA subunits suggesting good integrity of RNA. First strand cDNA was synthesized using one step RT-PCR Kit. The PCR was performed and the product was subjected to agarose gel electrophoresis which yielded targeted amplification of 1107 bp, 1567 bp and 486 bp amplicon for CYP3A, CYP2HI and p-actin genes, respectively. β-actin (house keeping gene) was used as an internal control for normalization of CYP3A and CYP2H1 gene transcripts. Quantitative RT-PCR was done to quantify gene expression level of CYP3A and CYP2H1 genes. Four end points were selected for sample dropping at 26th, 31st 36rd and 41st cycles to perform Quantitative RT-PCR. The quantity of expressed genes was detected by Gene tool software using 1 kb DNA ladder having concentration of 7.1 ng/0.5 μl at 500 bp as reference. Relative expression ratio of CYP3A and CYP2H1 genes was calculated by Relative Expression Software Tool (REST), It was found that CYP3A is up regulated by a factor of 1.271 and 1.2 in Bantam and White Leghorn chicks, respectively and down regulated by a factor of 11.385 in Bantamized White Leghorn chicks. In Bantamized White Leghorn and White Leghorn chicks CYP2H1 gene was down regulated by factor 1.68 and 1.3 respectively, but up regulated by a factor of 1.126 in case of Bantam chicks. The PCR efficiency ranged from 1.4 to 1.9, 1.36 to 1.8 and 1.36 to 4.4 for CYP3A, CYP2H1 and P-actin genes, respectively in Bantam, Bantamized White leghorn and White Leghorn chicks.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810054869
dc.keywordsComparative Evaluation of Dexamethasone Induced CYP3A, CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn, White Leghorn Chicksen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.research.problemComparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicksen_US
dc.subVeterinary Pharmacology and Toxicologyen_US
dc.subjectVETERINARY PHARMACOLOGY AND TOXICOLOGYen_US
dc.subjectA Studyen_US
dc.themeComparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicksen_US
dc.these.typeM.V.Sc.en_US
dc.titleComparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicksen_US
dc.typeThesisen_US
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