Seed vigour assessment in kasuri methi (Trigonella corniculata L.)
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Date
2013
Authors
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Journal ISSN
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Publisher
CCSHAU
Abstract
Kasuri methi (Trigonella corniculata L.) is a leguminosae seed spice crop. The two seedlots
each of six genotypes viz. GC-208, GC-209, HM-538, HM-557, KM139-1, KMHosiyarpur
of kasuri methi were evaluated for assessing vigour potential. Observations
were recorded on test weight (g), seed density (g/cc), standard germination(%), seedling
length (cm), seedling dry weight (mg), vigour index-I, vigour index-II, accelerated ageing
test (%), electrical conductivity (µS/cm/g), dehydrogenase activity test (OD g-1ml-1), field
emergence index (FEI), mean emergence time (MET, days) and total seedling establishment
(SET, %). Results revealed that the highest germination percentage (77.33%) was exhibited
in a two-way interaction TP, 200C of substrate and temperatures. Final seedling evaluation
was done on 10th day as the number of normal seedlings became stable. It was concluded
that the seeds of this crop may be tested for standard germination in TP substrate at 200C
and finally the seedlings may be evaluated on 10th day. After 96 hours (4th day) of
accelerated ageing, the germination decreased maximum from 64.66 to 48.66 percent (16%)
and declined below 50 percent. Therefore, the seeds conditioned at 400C, 100% RH for 96
hours were found optimal conditions for ageing technique in this crop. On the basis of
various vigour parameters studied, the genotype KM-Hosiyarpur was found more vigorous
followed by KM139-1 and HM-538. The lot L1 (fresh seed lot) of each genotype was found
superior than lot L2 (one year old seed lot) in terms of laboratory and field parameters
which determine the planting value of a seed-lot. The standard germination test was found
significantly and positively associated with test weight, vigour indices, field emergence
index whereas negatively with electrical conductivity (EC) test and mean emergence time
(MET). Total seedling establishment showed significant positive correlation with seed
density, field emergence index and negative with mean emergence time. Thus, above
mentioned vigour parameters may be given due consideration in prediction of germination
percentage and field emergence potential of seed-lots/genotypes in kasuri methi crop. The
FEI and MET were found negatively associated with each other. This indicated that the
genotype/seed-lot which emerged quickly under field conditions. The SG and SET were not
found significantly correlated with each other. This indicated that the germination capacity
did not truly depicted the seedling establishment under field condition.
Description
Keywords
Kasuri methi, Vigour, Standard germination, Accelerated ageing, Electrical conductivity, Field emergence index, Mean emergence time, Total seedling establishment