OCCURRENCE AND CLINICO-THERAPEUTIC STUDIES ON FELINE INFECTIOUS ANAEMIA
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Date
2017
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
The present study was designed to investigate the clinical, haemato-biochemical profile and risk factors associated with feline mycoplasma infection and its molecular diagnosis. Hundred anaemic cats presented at UVH, Kokkalai and Mannuthy during January to December 2016, were formed the subjects for study. Peripheral blood smears, were collected from each animal and subjected to Romanowsky staining for initial screening and further confirmed by acridine orange staining. The presence of small coccoid organism in the periphery of red blood cells were identified in blood smears of eleven out of hundred anaemic cats, suggestive of haemotropic Mycoplasmas. Most frequent clinical signs noticed in affected cats were pale mucous membranes, flea infestation, reduced appetite, pyrexia, splenomegaly and lymphadenopathy. Haematological studies revealed leucocytosis with granulocytosis, reduction in RBC count, haemoglobin and platelet count. Biochemical alterations such as Hyperglobulinaemia, elevated creatinine and ALT were noticed in cats with haemotropic mycoplasmosis. More number of cases were observed in summer season and among adult male non - pedigree cats with outdoor access. Whole blood samples were subjected to DNA isolation followed by polymerase chain reaction targeting 16s rRNA gene. Twenty four out of hundred animals were found to be positive for genus specific PCR assay and twenty three animals were found to be positive for Candidatus Mycoplasma haemominutum and only one cat was positive for Mycoplasma haemofelis. Additionally a multiplex PCR assay was also standardised for the simultaneous detection of both mycoplasma species. The infected animals were treated successfully with parenteral administration of oxytetracycline followed by oral doxycycline or doxycycline alone depending upon condition of animals. Response to the treatment was assessed by resolution of clinical signs and absence of organism in the blood smear.
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KVASU REFERENCE CITATION