Tissue culture studies in Alfalfa (Medicago sativa Linn.)
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Date
2011
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Publisher
CCSHAU
Abstract
In the present investigation attempts were made to develop an efficient protocol for in vitro
plant regeneration in Medicago sativa L. Initiation of callus formation from hypocotyl explants was
observed within 13-15 days of culture. Initiation of callus formation from cotyledons explants was
observed within 13-15 days of culture. Maximum callus formation was obtained in R3 medium
containing MS basal + NAA (1.0 mg/l) + Kinetin (0.3 mg/l). Maximum number of shoots was obtained
in R12 medium containing MS basal + NAA (1.0 mg/l) and Kinetin (0.5mg/l). MS basal medium was
used for roots induction, roots were observed after 14 days with very good quality.In media R2 (MS
basal + NAA 0.1mg/l + 2 IP 1.0 mg/l) and R8 (MS basal +NAA 2.0 mg/l), when shoots were left little
longer rooting occurred. After transplantation, 73.3% plants survived and these plants looked normal
with no morphological changes in leaf structure and plant type.Thus in the present study, regeneration
protocol in Medicago sativa L variety T9 has been developed using hypocotyls and cotyledon explants.
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Keywords
Planting, Regeneration, Lucerne, Vegetative propagation, Biological phenomena, Medicago sativa, Biological development, Auxins, Tissue culture, Embryonic development