ANTICANCER ACTIVITY OF Hordeum vulgare AND Pergularia daemia IN TRIPLE NEGATIVE BREAST CANCER CELLS

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dc.contributor.advisorBibu John Kariyil
dc.contributor.authorAKHIL G.H.
dc.date.accessioned2020-09-23T06:34:05Z
dc.date.available2020-09-23T06:34:05Z
dc.date.issued2019
dc.description.abstractThe present study was aimed to evaluate the anticancer activity of methanol extract of germinated seeds of Hordeum vulgare (Barley) and leaves of Pergularia daemia (Veliparuthi) in MDA-MB-231 cell line. Qualitative phytochemical screening showed the presence of steroids, glycosides, diterpenes, triterpenes and saponins in H. vulgare whereas tannins and flavonoids were additionally detected in P. daemia. The RP-HPLC analysis showed the presence of hordenine and lupeol in H. vulgare and P. daemia respectively. In vitro antioxidant activity of the extracts by DPPH and superoxide anion radical scavenging assays showed a significant (p<0.05) concentration-dependent antioxidant activity for both the extracts. The IC50 values for DPPH assay were found to be 112.75±5.74 and 100.67±7.9 μg/mL for H. vulgare and P. daemia respectively and for superoxide radical scavenging assay, the IC50 values were found to be 28.90±1.85 and 39.63±1.41 μg/mL for H. vulgare and P. daemia respectively. Cytotoxicity assay revealed that both the extracts produced potent cytotoxicity with IC50 values of 41.28±0.30 and 35.95 35.95±3.57 μg/mL for H. vulgare and P. daemia respectively. Morphological evaluation produced similar cytotoxic morphological changes in cells treated with standard hordenine and lupeol when compared with their respective extracts exhibiting morphological alterations such as reduction in cell population, presence of apoptotic bodies, cell shrinkage and vacuole formation. Both the extracts produced early apoptosis as evident by acridine orange/ethidium bromide staining characterised by yellowgreen fluorescence. Hoechst staining revealed that both the extracts altered nuclear morphology indicative of apoptosis with features such as nuclear marginalisation and fragmentation. Mitochondrial membrane potential (MMP) assessed by JC-1 staining revealed that H. vulgare partially altered MMP whereas P. daemia completely altered MMP. The results of the comet assay revealed that P. daemia alone was able to cause DNA damage significantly (p<0.01). The results of western blotting showed a significant (p<0.01) downregulation of Bcl-2 expression in P. daemia treated cells whereas caspase-8 expression was significantly (p<0.01) upregulated in H. vulgare treated cells. Thus, the study revealed that H. vulgare and P. daemia produced apoptosis by extrinsic and intrinsic pathway respectively.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151820
dc.keywordsANTICANCER ACTIVITY Hordeum vulgare AND Pergularia daemia TRIPLE NEGATIVE BREAST CANCER CELLSen_US
dc.language.isoEnglishen_US
dc.pages95en_US
dc.publisherCollege of Veterinary and animal Science,Mannuthyen_US
dc.research.problemANTICANCER ACTIVITY OF Hordeum vulgare AND Pergularia daemia IN TRIPLE NEGATIVE BREAST CANCER CELLSen_US
dc.subVeterinary Pharmacology and Toxicologyen_US
dc.themeANTICANCER ACTIVITY OF Hordeum vulgare AND Pergularia daemia IN TRIPLE NEGATIVE BREAST CANCER CELLSen_US
dc.these.typeM.V.Sc.en_US
dc.titleANTICANCER ACTIVITY OF Hordeum vulgare AND Pergularia daemia IN TRIPLE NEGATIVE BREAST CANCER CELLSen_US
dc.typeThesisen_US
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