CLINICO-THERAPEUTIC STUDIES ON BACTERIAL MASTITIS IN GOATS
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Date
2005
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
The lactating does in the University goat and sheep farm were screened for
subclinical mastitis once in three months using the California mastitis test. The
occurrence of subclinical mastitis was found to be 30.2 per cent. Statistical analysis
showed no significant association between occurrence of subclinical mastitis and
teat length whereas there was significant association between distance from teat tip
to floor. Among 642 samples screened 194 samples were found to be positive by
CMT.
The arithmetic mean cell counts for each CMT score ranged between 0.736 ± 0.033 xlO^ and 20.417 ± 0.851 xlO^ cells/ml. Among CMT positive samples
MWST and MAMP detected 62.89 per cent and 43.29 per cent as positive for
subclinical mastitis. Comparison of screening tests revealed that significant
positive correlation existed among the four tests namely CMT, MWST, MAMP and
see. Comparison with culture results showed that score '3' of CMT score '3+' of
MWST and grade 3 of MAMP reaction detected the maximum positive cases. CMT
scores and SCC in bacteriologicaly positive samples showed significant association.
Among the TIST positive milk samples 20 (44.44 per cent) were culture positive.
Staphylococcus aureus was the most predominant isolate in both clinical
and subclinical caprine mastitis. In vitro antibiotic sensitivity pattern revealed that
chloramphenicol was the most sensitive antibiotic followed by ceftriaxone and
ciprofloxacin. The isolated pathogens showed maximum resistance to sulpha.
Comparison of treatment trials in 24 clinical goat mastitis cases using ceftriaxone
and ciprofloxacin with 12 animals in each group revealed that clinical and
bacteriological cure was better in the case of ciprofloxacin. Clinical and
bacteriological cure was comparatively less in gangrenous mastitis cases.
Eighteen Staphylococcus isolates from clinical mastitis cases and 23
Staphylococcus isolates from subclinical cases were typed by RAPD fingerprinting.
Twelve different genotypes were obtained among which genotype c predominated
in clinical mastitis whereas in subclinical cases b and i were the common
Staphylococcal genotypes. Clinieal and bacteriological cure rates were 100 per cent
for RAPD type 1 in the ceftriaxone treated group and genotypes c and i in the
ciprofloxacin treated group of animals. A possible relationship regarding the
genetic make up of the different Staphylococcal isolates was elucidated from the
phylogenetic tree generated from the RAPD fingerprints.
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