Molecular characterization and genetic divergence analysis in date palm (Phoenix dactylifera L.) using RAPD markers
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Date
2007
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Publisher
CCSHAU
Abstract
Date palm (Phoenix dactylifera L.) is a long living dioecious
evergreen with 2n=36. It has the distinction of being one of the
oldest fruit trees in the world. It is an important plantation crop
due to nutritive quality of its fruit. This study was undertaken to
assess polymorphism among different date palm genotypes
(including male and female genotypes). Molecular polymorphism in
40 date palm genotypes (10 male and 30 female) was assessed by
using 29 RAPD primers. A total of 223 amplicons were observed out
of which 3 were monomorphic and 220 were polymorphic. Thenumber of amplified DNA bands varied between 2 and 17 with an
average of 7.69±0.70 bands per primer. Average polymorphism
across the 40 genotypes was 99.12±0.62. Three primers were
identified which produced unique alleles capable of differentiating
female l genotypes of Medjool from rest of the genotypes.
Analysis of the polymorphism profile generated using
NTSYS-PC software, grouped the 40 genotypes into 2 major
clusters and thirteen sub clusters. Z2 and M6 were out grouped
showing them to be genetically most diverse among all genotypes
used in present study. Based on ‘Simqual’ sub programme, genetic
similarity was calculated. It ranged from 0.28 to 0.93 indicating
moderate genetic variability among the genotypes. The average
similarity of male plants with all the thirty female plants was found
to be 0.7. Thus RAPD markers detected a high level of
polymorphism and the fingerprint data generated in this study can
be used for the identification of Medjool variety of date palm.
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Keywords
Datepalm, RAPD, polymorphism, Phoenix dactylifera