ISOLATION AND MOLECULAR DETECTION OF Mycoplasma capricolum subsp. capripneumoniae ASSOCIATED WITH RESPIRATORY TRACT INFECTION IN GOATS
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Date
2018
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
The present research was undertaken to detect the presence of mycoplasmas
associated with respiratory infection in goats with a preference to Mccp, the exact
cause of CCPP. Ninety-six nasal swabs were collected from goats with respiratory
infections from selected veterinary hospitals and farms in Ernakulam, Thrissur,
Palakkad, Malappuram and Wayanad districts. Postmortem samples including
lungs, mediastinal lymphnodes and pleural fluid were collected from 10 animals
with pneumonic lesions brought for postmortem examination in the Department of
Veterinary Pathology. Nucleic acid based techniques like PCR and conventional
methods like isolation were employed for the detection of mycoplasmas. Out of
the 106 samples tested, 52 (49 per cent) were found to be positive by 16S rRNA
genus specific PCR. Among the Mycoplasma genus positive samples, one was
found to be positive for Mycoides cluster, Mycoides group and Mmc specific PCR
and was confirmed as Mycoplasma mycoides subsp. capri by nucleotide
sequencing. None of the samples were found to be positive in Mccp specific PCR.
A representative sample from each of the 16 flocks was subjected to nucleotide
sequencing and the results revealed that animals in 10 flocks were infected with
M. ovipneumoniae, four with M. conjuctivae, one with M. capri and one with M.
agalactiae. On inoculating samples on to PPLO medium (modified Hayflick’s
medium), two samples produced typical fried egg like colonies on agar plate and
was confirmed as Mycoplasma by 16S rRNA genus specific PCR. Later, it was
confirmed as M. agalactiae by nucleotide sequencing. The isolation rate was
found to be 3.84 per cent.
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