Studies on Phenotypic Morphometric Traits and HSP-70 Gene Polymorphism in Local Donkeys of Akola
| dc.contributor.advisor | Bankar, P. S. | |
| dc.contributor.author | Shinde Shubhangi Sanjay | |
| dc.date.accessioned | 2023-11-02T17:48:02Z | |
| dc.date.available | 2023-11-02T17:48:02Z | |
| dc.date.issued | 2023-04-03 | |
| dc.description.abstract | This study documented the morphometric, phenotypic traits and breeding practices of local donkeys in Akola district, Maharashtra. Total 86 local donkeys were subjected for recording and socioeconomic profile of 96 donkey farmers was also surveyed using preformed questionnaire. Additionally, genetic polymorphism of the HSP-70 gene in donkeys were investigated. 50 unrelated Kaikadi donkeys were analyzed for genetic polymorphism using PCR RFLP of the HSP-70 gene (169 bp) with BsaAI restriction enzyme. The donkeys were of medium sized with grey coat colour prominently. Phenotypic characteristics of the donkeys, such as black eyes, eyelids and hooves, a light grey muzzle, and dark brown tail switch were also noticed. Nasal bone was concave and straight. The overall mean values for various morphometric traits (cm) recorded in adult local donkeys (n=86) belonging to both sexes exhibited mean values for Body length (BL), Height at wither (WH), Height at croup (HC), Height at knee (HN), Height at hock (HH), Arm length (AL), Dis. bet. withers to croup (WC) and Dis. bet. croup and tail head (CT), Chest girth (CG), Paunch girth (PG), Tail length (TL), Face length (FL), Face width (FW), Dis. bet. eyes (ED), Ear length (EL), Ear width (EW) and Body weight (BWT) were 73.43±0.32 cm, 91.20±0.58 cm, 93.44 ±0.60 cm, 31.39±0.14 cm, 35.19 ±0.14 cm, 84.52 ±0.61 cm, 55.11 ±0.48 cm, 17.94±0.08 cm, 97.31±0.54 cm, 101.29 ±0.54 cm, 51.90 ±0.32 cm, 41.73±0.35 cm, 19.24 ±0.27 cm, 9.94 ±0.11 cm, 21.00 ±0.17 cm, 10.03 ±0.16 cm and 58.66 ±0.90 kg, resp. The Least square analysis of variances exhibited effect of non-genetic factors viz. age, sex and location etc. on various morphometric traits. Kaikadi donkeys were primarily reared by Kaikadi community, belonging to NT category with medium family size, mostly illiterate and landless and labour. All the donkey farmers surveyed were below poverty line. The families kept donkeys as their traditional business for draft or pack purposes. All the donkey farmers preferred for Natural breeding using their own jenny and no specific criteria for selection of male for breeding. The donkeys were kept in open (61.46%) and Kaccha (97.79%) shed, which located near residence (92.71%). The PCR-RFLP of HSP-70 gene (169 bp) using synthetic primer Forward primer-5-ACATGGCAAGGTGGAGATCA-3 and Reverse primer-5’-GAACTTGCGTCCGATCAGTC-3’) and digesion with BsaAI restriction enzyme exhibited uncut fragment of 169 bp i.e. monomorphic AA genotype for all the studied samples. Thus, the frequency of observed genotypes AA, AB and BB was estimated as 1, 0 and 0 respectively and complete absence ‘B’ allele and fixation of allele A in population in studied local donkey of Akola district. The study found that the Kaikadi donkey population in Akola district was of small size and has unique physical characteristics compared to other donkey populations in India. The HSP-70 gene locus studied was found to have a fixed AA genotype in the donkey population. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810200212 | |
| dc.keywords | Animal Genetics | |
| dc.keywords | Breeding | |
| dc.keywords | Phenotypic | |
| dc.keywords | Morphometric Trait | |
| dc.keywords | Gene Polymorphism | |
| dc.keywords | Local Donkey | |
| dc.language.iso | English | |
| dc.pages | 115 | |
| dc.publisher | MAFSU, NAGPUR | |
| dc.sub | Animal Genetics and Breeding | |
| dc.theme | Gene Polymorphism in Local Donkeys of Akola | |
| dc.these.type | M.V.Sc. | |
| dc.title | Studies on Phenotypic Morphometric Traits and HSP-70 Gene Polymorphism in Local Donkeys of Akola | |
| dc.type | Thesis |