Analysis of Hariana Cow Urine for Presence of Various Proteins/Peptides in Comparison to Murrah Buffalo
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Date
2016
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Publisher
LUVAS
Abstract
Modern proteomics technique offers an exciting opportunity in solving some of the most challenging problems of livestock sector, including development of diagnosis based on urinary protein profiling. The present study intended to identify proteins, which may be novel to Hariana cow and Murrah buffalo urine and can subsequently be used for the purpose of disease detection. The urine samples were subjected to diafilteration and TCA precipitation followed by 2-dimensional gel electrophoresis and densitometric analysis in PD Quest software, which revealed the presence of at least 20, 2-D gel spots exhibiting specific appearance in a specific animal. Some of the Identified spots, which are documented to have role in many body functions including body defence, activation of cytoskeleton system, maintenance of muscular activity, suppression of tumour, biomarker of certain diseases, treatment of hypertension, apoptosis regulation includes the adenomatous polyposis coli (APC), vinculin proteins, cystatin-M precursor, melanoma inhibitory activity protein 3, epoxide hydrolase 2, bcl-2-like protein 11. The current work shows light on presence of some of the biologically active substances in the urine which could explain about its medicinal value. Considering that proteomics in animal research is still in its infancy, it is a long way before practical applications of such experiments could actually be realized.