Characterization of High Grain Weight Introgression Lines Derived From Wild Tetraploid Triticum dicoccoides (AABB) In Wheat.

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dc.contributor.advisorMavi, G.S.
dc.contributor.authorParamjot Singh
dc.date.accessioned2023-12-29T15:12:36Z
dc.date.available2023-12-29T15:12:36Z
dc.date.issued2023
dc.description.abstractBread wheat (Triticum asetivum L., AABBDD, 2n= 6x= 42) belongs to family Poaceae, one of the most critical food crops. As the population is increasing day by day, therefore demand of food is increasing. The challenge before the scientist is to increase the production of food crops as the land for cultivation is decreasing due to population boom. So, scientists are exploring the wild accessions of wheat as these have wide variation for the traits related to yield enhancement. Yield is the complex quantitative trait, the genetic × environmental (G × E) interaction has significant impact on it. In contrast to its seed weight, which is formed of individual grain size, morpho-metric components of grain area, length, and width, is shown to be more stably inherited than final yield. T. diccocoides represents great genetic variation for grain length, which could be used as an important resource in the wheat improvement. In the current study, 13 wild accessions of T. diccocoides available with PAU were used to cross with six hexaploid wheat genotypes, namely PBW723, WL711NN, PBW621, PBW343, HD2967 and WH1105. Selections were made for plants that were showing high grain parameters like grain length, grain width and thousand grain weight and these selected plants were advanced up to BC2F6 generation. In BC2F5 and BC2F6, phenotypic data of BILs were taken related to grain length, grain width, thousand grain weight, plant height, spike length, spikelet per spike. Analysis of variance revealed the presence of variability for all the traits. The broad sense heritability and genetic advance over mean revealed that traits spike length and thousand grain weight have high broad sense heritability and genetic advance over mean, so selections for these traits will be performed from the BILs. Further, correlation study revealed that grain length and grain width have significant and highly positive correlation with thousand grain weight. The introgression profiling was done for the BILs. For profiling of the BILs 214 SSR markers were used. In each chromosome around 15 to 20 markers were applied that were present along the whole chromosomes. The 71 markers were polymorphic on parents and these were amplified on BILs, 40 markers were found polymorphic. The comparison was made with BILs with low and high grain length and grain width. Majority of introgressions were found on the B genome, primers WMC336, WMC757, WMC128, WMC673, WMC597, GWM644, WMC459 and WMC76 were found introgressed in the BILs that were having high grain length and grain width. The protein content of the BILs was varying from 6% to 15.44%. BILs TD-8, 14, 15, 20 were selected as best performing BILs after analysing both years data.
dc.identifier.citationParamjot Singh (2023). Characterization of High Grain Weight Introgression Lines Derived From Wild Tetraploid Triticum dicoccoides (AABB) In Wheat. (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810205016
dc.keywordsWheat
dc.keywordsT.diccocoides
dc.keywordsGrain length
dc.keywordsGrain width
dc.keywordsSSR
dc.keywordsIntrogression
dc.language.isoEnglish
dc.pages90
dc.publisherPunjab Agricultural University
dc.research.problemCharacterization of High Grain Weight Introgression Lines Derived From Wild Tetraploid Triticum dicoccoides (AABB) In Wheat.
dc.subGenetics and Plant Breeding
dc.themeCharacterization of High Grain Weight Introgression Lines Derived From Wild Tetraploid Triticum dicoccoides (AABB) In Wheat.
dc.these.typeM.Sc
dc.titleCharacterization of High Grain Weight Introgression Lines Derived From Wild Tetraploid Triticum dicoccoides (AABB) In Wheat.
dc.typeThesis
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