IN VITRO REGENERATION OF Psoralea corylifolia AND Tinospora cordifolia- ANTICANCEROUS AND ANTILEPROTIC PLANTS
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Date
2014-05
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FOREST BIOLOGY AND TREE IMPROVEMENT DEPT., ASPEE COLLEGE OF HORTICULTURE AND FORESTRY, NAVSARI AGRICULTURAL UNIVERSITY, NAVSARI
Abstract
The present investigation on “IN VITRO
REGENERATION OF Psoralea corylifolia AND Tinospora
cordifolia-ANTICANCEROUS AND ANTILEPROTIC
PLANTS” was carried out at Plant Tissue Culture Laboratory,
Department of Forest Biology and Tree Improvement, ASPEE
College of Horticulture and Forestry, Navsari Agricultural
University, Navsari, Gujarat, India during 2012-14. In vitro
regeneration protocol for P. corylifolia was established 1) from
nodal and shoot tip explants 2) Direct regeneration through
adventitious shoot bud formation on root explants. However for T.
cordifolia, regeneration protocol from nodal segment has been
established.
For In vitro regeneration of P. corylifolia from nodal
and shoot tip explants, the highest percent (86.70 %) of shoot bud
regeneration was noticed on MS medium containing 6.98 µM Kin
for nodal segment while 87.83 % shoot bud regeneration was
observed for shoot tip. MS medium supplemented with 6.98 µM
Kin produced maximum number of shoots (6.67 shoots/explant),
shoot length (5.67 cm) and number of leaves (7.0) for nodal
explants. Same treatment produced 5.67 number of shoot with
average 5 cm length and 6.0 number of leaves for shoot tip
explants. Large number of culture showed vitrification in both kind
of culture. MS media fortified with Kin showed comparative less
vitrification. Maximum hyperhydric shoots were produced by TDZ
even at low concentration. Phloroglucinol reduces hyperhydricity
drastically and positively increases the explants response to shoot
bud induction in both types of culture. Basal MS + 6.98 µM Kin +
39.7 µM PG evinced maximum effect on shoot bud multiplication
(11.67shoot/explant in nodal explants and 9.83shoot/explant in
shoot tip culture). The highest percent of root induction (82.2%)
was observed on half-strength MS medium fortified with 4.92 µM
IBA and 158.6 µM PG within 10 days with maximum number of
roots (4.5 roots/shoot) and average root length of 4.03 cm. Rooted
explants successfully hardened in Soil: Vermicompost: Vermiculite
(1:1:1) with 100% success
For direct regeneration through adventitious shoot bud
formation on root explants in Psoralea corylifolia, effect of
different PGRs (Kin, BA, and NAA) on the percentage of
adventitious shoot induction and the number of shoot buds per root
explant was assessed. The maximum response (65.57 per cent)
observed in the media supplemented with 2.22 µM BAP and 6.98
µM Kin with average 39.67 number of shoot bud and 3.37 number
of elongated shoot. Elongation response was best (92.0 percent) in
MS+2.22 µM BAP +6.98 µM Kin media fortified with 2.89 µM
GA3 and 39.7 µM PG, producing mean 9.3 number of elongated
shoot with average 4.6 cm length. Anatomical study showed that
the origin of shoot bud were directly from pericycle of root.
Elongated shoots were carefully excised and rooted on basal
MS+4.92 µM IBA+158.6 µM PG, with 92 per cent rooting. Rooted
explants successfully hardened in Soil: Vermicompost: Vermiculite
(1:1:1) with 100% success
In T. cordifolia, within a week of nodal explants
culture, axillary bud in nodal segments sprouted in all the media
investigated including basal MS media without any growth
regulators. Maximum response (52.2 per cent) evinced by MS
media supplemented with 6.98 µM Kin. The same media produced
maximum response for multiple shoot formation out of total shoot
bud sprouted fostering average 3 shoots with 3.9 cm shoot length
and 4.2 number of leaves per shoot. Effect of PG also investigated.
Basal MS + 6.98 µM Kin + 79.4 µM PG increased shoot bud
induction from 52.2 per cent to 84.8 per cent and multiple shoot
production per cent from 12.9 to as high as 60.3 percent and also
supported maximum number of shoot per explants (7.5). The same
treatment promoted highest axillary shoot proliferation in terms of
shoot length (3.9 cm) and number of leaves per shoot (4.3).
Maximum rooting response observed in ½ MS medium
supplemented with 7.4 µM IBA and 793.7 µM PG with 81.1 %
responding cultures, took just 8.7 days to sprout and produced
maximum number of roots (3.2) with an average length (3.1 cm).
Rooted explants successfully hardened in Soil: Vermicompost:
Vermiculite (1:1:1) with 100% success.
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