Characterisation of transformed sugarcane carrying anti-fungal β-1,3-glucanase gene

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dc.contributor.advisorSandhu, Jagdeep Singh
dc.contributor.authorNayyar, Shivani
dc.date.accessioned2017-08-20T09:39:45Z
dc.date.available2017-08-20T09:39:45Z
dc.date.issued2016
dc.description.abstractThe present study entitled “Characterisation of transformed sugarcane carrying anti-fungal β-1,3-glucanase gene” was carried out to characterize in planta transformed sugarcane plants. The objective of this study was to induce red rot resistance caused by C. falcatum. The in planta transformed T0 putative transgenic plants (10) were propagated through plant to row progenies leading to formation of 295 axillary bud setts, out of which 127 setts sprouted representing the clonal generation 1 (CG1). The PCR analysis on T0 putative transgenic plants of CG1 (127 clones) revealed the amplification of 1764 bp β-1,3-glucanase transgene in six putative transgenic plants. The RT-PCR pointed towards the differential expression of transgene in all the six plants and the relative β-1,3-glucanase transgene expression by qRT-PCR confirmed that three plants had 3.197, 4.280 and 4.438 fold increase in transgene expression as compared to non-transformed plant. Bioassay for red rot on six qRT-PCR positive plants with Cf08 pathotype demonstrated that three plants showed resistance against red rot and with Cf09 pathotype demonstrated that two plants showed moderate resistance against red rot. The CG2 plant analysis through qRT-PCR revealed similar relative transgene expression in the clones suggesting absence of chimerism.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810029146
dc.keywordsSugarcane, Agrobacterium mediated in planta transformation, β-1,3-glucanase, PCR, RT-PCR, qRT-PCR, Bioassay, Disease Resistanceen_US
dc.language.isoenen_US
dc.pages59en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemCharacterisation of transformed sugarcane carrying anti-fungal β-1,3-glucanase geneen_US
dc.subAgricultural Biotechnologyen_US
dc.subjectnullen_US
dc.themeCharacterization of transformed sugarcaneen_US
dc.these.typeM.Scen_US
dc.titleCharacterisation of transformed sugarcane carrying anti-fungal β-1,3-glucanase geneen_US
dc.typeThesisen_US
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