Physiological, biochemical and molecular parameters of salt tolerance in different genotypes of barley (Hordeum vulgare L.)
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Date
2019
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Punjab Agricultural University, Ludhiana
Abstract
In the present investigation, the influence of salt stress on physiological, biochemical and
molecular parameters in barley (Hordeum vulgare L.) genotypes at different salinity levels
was studied. Seeds of ten barley genotypes (BL1400, BL1314, PL890, PL807, RD2552,
PL751, BL1430, PL426, BL1375 and BL1367) were treated with different salt concentrations
(0, 25, 50, 75, 100, 125, 150, 175 and 200 mM NaCl). The salinity stress has deleterious
effect on seed germination and seed vigour parameters. Increased salinity caused a significant
reduction in percentage of germination and seed germination rates. Also, a significant
reduction in root and shoot length of seedlings was recorded. Seedling biomass (fresh and dry
weight) declined in stressed seedlings as compared to control. Among all salt concentrations
effect of salt stress was more differentiable at 75mM and 100mM. Therefore, these salinity
levels were selected to test the effect of salt stress on biochemical parameters. All
biochemical parameters viz. total soluble sugar content, total soluble protein content and
activity of antioxidant enzymes (catalase, peroxidase and superoxide dismutase) increased
with increased salt stress. Also, molecular studies were conducted on comparatively sensitive
and comparatively tolerant genotype under selected salt concentration levels.
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