Isolation and characterization of rhizobia infecting fenugreek (Trigonella foenumgraecum L.)

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dc.contributor.advisorKukreja, Kamlesh
dc.contributor.authorPoonam Rani
dc.date.accessioned2016-09-15T09:34:58Z
dc.date.available2016-09-15T09:34:58Z
dc.date.issued2014
dc.description.abstractNutrient enrichment of soil by nitrogen fixing symbiotic bacteria present in legumes has been known for centuries. Fenugreek (Trigonella foenumgraecum L.) is one of the oldest herbs and annual plant in the family Fabaceae and is cultivated worldwide as a semi-arid crop. The largest producer of fenugreek in the world is India. Rajasthan produces the lion's share of India's production, accounting for over 80% of the nation's total fenugreek output. The most common nodule forming bacteria associated with fenugreek is the Gram negative, aerobic, nonsporulating, rod shaped bacterium, Rhizobium meliloti. In the present study, a total of 48 rhizobial isolates were obtained from nodule samples of fenugreek crop collected from farmer’s field of different districts (Sirsa, Hisar and Karnal) of Haryana state. Authenticity of rhizobia was done by plant infection test under sterilized conditions. All the rhizobial isolates formed nodules. On the basis of nodulation in sterilized cups, 31 fenugreek rhizobial isolates were characterized for IAA production, siderophore production, P- solubilization, temperature tolerance and bacteriocinogenicity. Out of 31 rhizobial isolates, 22 were IAA producers. IAA production ranged from 24.0 to 62.6 μg ml-1. Eleven were siderophore producers and their relative siderophore production ability varied from 1.25 to 1.87. Two rhizobial isolates were phosphate solubilizers and six rhizobial isolates produced bacteriocin. All the isolates were able to grow at 20, 25, 30oC; however few isolates showed diminished growth at 20oC. Plant growth promoting efficiency of these rhizobial isolates was assessed under pot house conditions and it was found that all the inoculated treatments were better than the uninoculated control. Nodule fresh weight, plant height, plant biomass and shoot nitrogen in plants increased significantly with the inoculation of FS12, FS15, FH36, FK23 and FK35 rhizobial isolates. Nodule fresh weight was recorded highest in FS12 (284 mg plant-1) followed by FS15 (282 mg plant-1) and FK36 (247 mg plant-1). Shoot dry weight was observed highest in FK35 (2.52 g plant-1) followed by FH36 (2.46 g plant-1) and FK23 (2.28 g plant-1). Shoot nitrogen content was observed highest in FK35 (84.19 mg plant-1) followed by FH36 (83.72 mg plant-1) and FK23 (75.20 mg plant-1). Based on characterization and pot house study, best fenugreek rhizobial isolates were FS12, FS15, FH36, FK23 and FK35.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/76792
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subMicrobiology
dc.subjectPlanting, Root nodulation, Productivity, Biological development, Bacteria, Rhizobium, Nitrogen, Application methods, Biological phenomena, Cropsen_US
dc.these.typeM.Sc
dc.titleIsolation and characterization of rhizobia infecting fenugreek (Trigonella foenumgraecum L.)en_US
dc.typeThesisen_US
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