CHARACTERIZATION OF ENTEROPATHOGENIC ESCHERICHIA COLI FROM DOMESTIC ANIMALS FOR VIRULENCE AND COLONISATION FACTORS
| dc.contributor.advisor | Bhat, Mohd. Altaf | |
| dc.contributor.author | Shikha, Deep | |
| dc.date.accessioned | 2018-07-11T07:34:17Z | |
| dc.date.available | 2018-07-11T07:34:17Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | The study aimed at finding the occurrence of, enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) in different domestic animal species and to characterize the EPEC isolates with respect to their serogroup, accessary virulence attributes like bfpA, astA and ecpA genes and antibiotic resistance. Out of a total of 200 E. coli isolates (one isolate from each sample) from the faecal samples (calves=39, rabbits=24, dogs=38, pigs=22, sheep and goat=36 and poultry=41), 38 (19.00%) were detected as EPEC and rest 28 (14.00%) were identified as STEC. Five (12.82%) and 17 (43.58%) isolates from calves and 12 (33.33%) and 11 (30.55%) isolates from sheep were found to be EPEC and STEC, respectively. In case of rabbits, pigs and dogs, 6 (25.0%), 10 (45.45%) and 5 (13.15%) isolates of E .coli were detected as EPEC, respectively. None of these animals carried STEC. The most predominant EPEC serogroups were O88 (36.84%) and O118 (18.42%) followed by O149 (7.89%), O22 (5.26%) and O11 (5.26%). All the 38 EPEC (100%) isolates carried ecpA gene and only 18 (47.36%) isolates carried astA gene. None of the rabbit EPEC isolate carried the astA gene. Out of 38 EPEC isolates, only 5 (13.15%) isolates from dog carried the bfpA gene and therefore considered as typical EPEC and the other 33 (86.84%) isolates which didn’t carry the bfpA gene were designated as atypical EPEC. Antimicrobial sensitivity test of 38 EPEC isolates showed that resistance was most common to nalidixic acid (55.26%), kanamycin (42.10%) followed by streptomycin (42.10%), doxycycline hydrochloride (28.94%), ciprofloxacin (13.15%), tetracycline (13.15%). Surprisingly, all the 38 isolates were sensitive to ampicillin. | en_US |
| dc.identifier.citation | No. of references 220 | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810058991 | |
| dc.keywords | Antibiogram, EPEC, STEC, Multiplex PCR, Virulence factors | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 111 | en_US |
| dc.publisher | Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu | en_US |
| dc.research.problem | CHARACTERIZATION OF ENTEROPATHOGENIC ESCHERICHIA COLI FROM DOMESTIC ANIMALS FOR VIRULENCE AND COLONISATION FACTORS | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | CHARACTERIZATION OF ENTEROPATHOGENIC ESCHERICHIA COLI FROM DOMESTIC ANIMALS FOR VIRULENCE AND COLONISATION FACTORS | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | CHARACTERIZATION OF ENTEROPATHOGENIC ESCHERICHIA COLI FROM DOMESTIC ANIMALS FOR VIRULENCE AND COLONISATION FACTORS | en_US |
| dc.type | Thesis | en_US |