TOXICOPATHOLOGICAL EVALUATION OF COPPER IN BROILER CHICKEN
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Date
2015-09-18
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Kerala Veterinary and animal science university, Pookode
Abstract
The effects of different levels of dietary copper sulphate in broiler birds were evaluated by supplementing 200, 400, 600 and 800 ppm of copper sulphate along with basal diet from day eight to day thirty six. The growth parameters, histopathological changes on different organs, genotoxicity to blood cells and residual copper in different organs were estimated. The genotoxicity was screened using alkaline comet assay and residual copper in different organs were estimated using flame atomic absorbance spectrophotometer. The best growth performance was obtained with the supplementation of 200 ppm of copper. Copper above the level of 200 ppm resulted in reduced growth performance, gastro intestinal disturbances and hepatotoxicity in birds. On comet assay analysis a dose and time dependent increase in the comet tail length was observed which indicated genotoxic effect of copper on blood cells. The highest level of copper residue was found in liver. Kidney, muscle, heart and gizzard contained negligible concentration of residual copper. Evaluation of droppings for residual copper showed a logarithmic
increase with increased dietary supplementation. This investigation proved that copper above the level of 200 ppm was hepatotoxic and genotoxic to blood cells. Besides an increased copper excretion with increased supplementation of copper was observed. The residual effect of copper in edible organs were negligible. From this study it was observed that the best recommended level of copper in broiler birds as feed additive was 200 ppm.
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