Molecular characterization of poly hydroxy butyrate producing bacteria
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Date
2010
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Publisher
CCSHAU
Abstract
Polyhydroxybutyrates are the biopolymers and have a potential to be used as
biodegradable plastics to replace synthetic plastics. The present investigation has been
undertaken to isolate polyhydroxybutyrate producing bacteria and analyze the diversity among
them related with sequence of PHB synthase gene. The soil and water sample for isolation of
bacteria were collected from fourteen diverse sources. Out of hundred bacterial isolates forty
were found PHB producing after initial fluorescence based screening on Nile blue A containing
medium. By trying different combinations of carbon and nitrogen sources 2g glucose as carbon
source and 0.65g potassium nitrate was found as most suitable for intracellular PHB
accumulation. Six best PHB accumulating strains were selected and characterized for
optimization of PHB production media. The selected strains were producing 59-29% PHB of dry
weight of cell pellet. Different combinations of Agri-byproducts were tried as carbon source for
cost effective PHB production and 3g molasses was found as best carbon source for PHB
production (60% of cell dry weight).PCR amplification was performed by PHB synthase gene
specific primers with genomic DNA of selected strains. Diverse pattern of amplification was
observed which indicate towards diversity of phbC gene sequence. The gene specific band was
eluted and cloned in TA sequencing vector.
Description
Keywords
Bacteria, Productivity, Carbon, Genes, Ritrogen, Biological development, Dna, Acidity, Inorganic acid salts, Byproducts