EVALUATION OF HOST PLANT RESISTANCE AND CHARACTERIZATION OF VIRUS(ES) CAUSING LEAF CURL DISEASE OF CHILLI (Capsicum annuum L.) IN PUNJAB
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Date
2013
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Abstract
Chilli is known to be affected by forty five viruses, among them whitefly transmitted begomovirus of family Geminiviridae is considered to be the most destructive virus in terms of yield loss, causing leaf curl disease of chilli. In the present study, survey conducted in major chilli growing areas of Punjab showed that chilli leaf curl disease (ChiLCD) is prevalent in all the four districts surveyed with incidence ranging from 11 to 64%; maximum severity recorded in the months July-August in the districts Patiala and Sangrur. A total of 155 genotypes were screened under natural conditions and the identified resistant stocks were subjected to artificial inoculation. Symptom variability and mixed infections of begomovirus along with beta DNA satellite molecule was found in samples collected from field. Five begomoviruses viz., tomato leaf curl Jodyebpur virus (ToLCJV), tomato leaf curl Palampur (ToLCPV), tomato leaf curl New Delhi (ToLCNDV), tomato leaf curl Karnatka (ToLCKV) and papaya leaf crumple virus (PaLCrV) were found to be associated with ChiLCD in Punjab. Typical begomovirus symptoms were observed in artificially inoculated chilli lines after five weeks of inoculation. Three lines Surian 2010, Perennial and Japanese Loungi were promising against tomato leaf curl Joydebpur virus causing ChiLCD. Symptomatic and asymptomatic weeds showed presence of begomovirus when subjected to rolling circle amplification (RCA) + polymerase chain reaction (PCR), except Calatropis and Parthenium. Weeds are potential reservoir of tomato leaf curl Joydebpur virus (Abutilon and Ageratum), papaya leaf crumple virus (Cannabis), chilli leaf curl India virus (Ipomea and Amranthus) in Punjab.
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