DETECTION OF BOVINE CORONAVIRUS IN DIARRHOEIC CALVES
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Date
2018
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
The present research was undertaken to detect the presence of coronavirus in
faecal samples of calves with diarrhoea by RT-PCR and confirmation by TEM. One
hundred-and-four faecal samples of diarrhoeic calves below three months were
collected from farms and hospitals of Kerala Veterinary and Animal Sciences
University as well as from selected private farms located in Thrissur and Palakkad
districts. All the samples were screened for detecting bovine coronavirus (BCoV) by
RT-PCR with focus on the conserved nucleocapsid (N) gene of BCoV. Among 104
samples collected, two (1.92 per cent) were found to be positive by RT-PCR and both
were from calves in the age group 0-14 days. The PCR product was sequenced and
analysed using BLAST. The results showed one hundred per cent homology to BCoV
isolates from Croatia, Korea and France. The two samples positive in RT-PCR when
further examined by TEM, revealed typical morphology of BCoV, thus confirming the
presence of the virus. The phylogenetic analysis of the sequence of the BCoV was
carried out using MEGA 7 software. The evolutionary history inferred by maximum
likelihood method revealed that the sequence showed similarity to the sequences of
Croatia, Korea, France and several other BCoV isolates and divergence to avian
infectious bronchitis virus.
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