“STUDIES ON GROWTH PARAMETERS OF ENTOMOPATHOGENIC FUNGI AND ITS EVALUATION AGAINST Tetranychus urticae KOCH
| dc.contributor.advisor | Neelam, Joshi | |
| dc.contributor.author | Neha Sharma | |
| dc.date.accessioned | 2017-07-10T05:04:51Z | |
| dc.date.available | 2017-07-10T05:04:51Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | Eight fungal isolates were studied for the growth parameters on different substrates, effect of pesticides on fungal growth and pathogenicity against Tetranychus urticae. In the study of effect of pesticides on fungal growth at different concentrations radial growth of fungal isolates on the growth media supplemented with pesticides was recorded and was found that the radial growth of fungal isolates varied among genera and species. Among all fungal isolates, Metarhizium anisopliae MTCC 4104 @ 750 ppm was most compatible to propargite and showed 33.6 per cent growth reduction over control. In media supplemented with ethion, endosulfan and chlorpyriphos, Hirsutella thompsonii PDBC-1 caused 32.9 per cent @ 1000 ppm, 6.3 per cent @ 2500 ppm and 20.1 per cent @ 2500 ppm per cent growth reduction over control, respectively and was most compatible with these pesticides. Further, in all pesticides tested, radial growth varied in inverse proportion to the concentration. Lower the concentration, more is the radial growth. Seven different substrates were evaluated for growth parameters of all fungal isolates and variability of the mean conidial count, colony forming unit and biomass was observed both among substrates and various fungal isolates. Among the isolates, mean conidial count and colony forming unit was maximum in H. thompsonii PDBC-1 recording 26.79 x 106conidia/g and 26.6 x 106 CFU/g, respectively whereas Beauveria bassiana P isolate recorded maximum mean biomass production of 0.49 g. Among the substrates, sorghum recorded maximum mean conidial count and colony forming unit of 21.29 x 106conidia/g and 20.9 CFU/g, respectively whereas maximum biomass production was recorded in rice wash water (0.37 g). In bioassay studies against adult of T. urticae, the fungal isolate H. thompsonii PDBC-1 and Beauveria bassiana MTCC 6291 recorded 96.50 per cent mortality at 1011conidia/ml after seven days of treatment. Further in all treatments, higher concentration was significantly better than the middle and lower concentration. Similarly, H. thompsonii PDBC-1 recorded minimum LC50 value of 6.84 x 109, 1.20 x 107and 8.38 x 102 conidia/ml after 72, 120 and 168 hrs, respectively which was significantly better than all other isolates and was the most pathogenic isolate. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810024748 | |
| dc.keywords | Keywords: Beauveria bassiana, Fungi, Hirsutella thompsonii, Metarhizium anisopliae, pesticides Tetranychus urticae. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | PAU Ludhiana | en_US |
| dc.sub | Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | ENTOMOPATHOGENIC FUNGI | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | “STUDIES ON GROWTH PARAMETERS OF ENTOMOPATHOGENIC FUNGI AND ITS EVALUATION AGAINST Tetranychus urticae KOCH | en_US |
| dc.type | Thesis | en_US |