Biocontrol of fusarial wilt of bottle gourd
| dc.contributor.advisor | Narinder Singh | |
| dc.contributor.author | Ruchika, Ruchika | |
| dc.date.accessioned | 2020-11-13T05:12:51Z | |
| dc.date.available | 2020-11-13T05:12:51Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | The wilt of bottle gourd caused by Fusarium oxysporum is a serious disease in bottle gourd growing areas of Punjab. Rhizosphere soil samples were collected from bottle gourd fields of Ludhiana, Kapurthala and Bathinda districts of Punjab in order to analyze the antagonistic microflora. Five isolates of Trichoderma and three isolates of Pseudomonas were isolated and screened in vitro for their antagonistic activity against Fusarium oxysporum. Among the various isolates, two isolates of Trichoderma (T1 and T2) and one isolate of Pseudomonas (P1) showed good inhibition against Fusarium oxysporum under lab conditions. Trichoderma isolate T2 showed maximum inhibition of Fusarium oxysporum (76.33 % and 65.83%) in dual culture and volatile assay, respectively. On Molecular characterization the selected isolates T1 and T2 were identified as Trichoderma asperellum and isolate P1 was identified as Pseudomonas aeruginosa. The selected isolates were then applied as seed, soil and seed + soil treatments using 10g and 15g formulation of each antagonist to check their efficacy against Fusarium oxysporum in two different seasons (March and June) under field conditions. Maximum germination (91.67% and 87.50%) and maximum disease inhibition (92.31% and 86.67%) was found in case of chemical treatment followed by Trichoderma asperellum isolate T2 with a germination of 87.50 per cent and a disease reduction of 84.62 and 80.00 per cent in March and June, respectively. Maximum yield (235.99q/acre and 229.00q/acre) along with maximum total length (5.43 m and 4.83 m) was obtained in case of Trichoderma asperellum isolate T2 in March and June, respectively due to disease control and the plant growth promoting behaviour of the antagonist. The results of rhizospheric competence showed that enough propagules of the antagonists were present throughout the growing season to effectively manage the wilt pathogen. Talc based bioformulation of the selected antagonists showed maximum shelf life on storing at 0°C and packing in polythene bags. | en_US |
| dc.identifier.citation | Ruchika (2019). Biocontrol of fusarial wilt of bottle gourd (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810155006 | |
| dc.keywords | Bioformulation, Fusarium oxysporum, Pseudomonas aeruginosa, Rhizosphere competence, Trichoderma asperellum | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 81 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Biocontrol of fusarial wilt of bottle gourd | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.theme | Biocontrol of fusarial wilt of bottle gourd | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Biocontrol of fusarial wilt of bottle gourd | en_US |
| dc.type | Thesis | en_US |