GENDER IDENTIFICATION USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN PAPAYA (Carica papaya L.) var. Madhubindu
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Date
2016
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DEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND
Abstract
Experiments were conducted to screen and validate suitable male
specific markers in the identification of sex in papaya (cv. Madhubindu). From
the reported markers W11, T12, Napf and CPSM 90 showed detectable results
in sex determination and was used for further experimentation with routinely
used PCR method as well as loop mediated isothermal amplification (LAMP).
Using screened markers unknown samples were identified with 100%
efficiency of the marker used in predicting the sex type.
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