COMPARATIVE DIAGNOSTIC AND THERAPEUTIC PROTOCOL FOR THE MANAGEMENT OF DERMATOPHYTOSIS IN DOGS
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Date
2022-10-13
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE, WAYANAD, KERALA VETERINARY AND ANIMAL SCIENCES UNIVERSITY
Abstract
The study entitled “Comparative diagnostic and therapeutic protocol for the management of dermatophytosis in dogs” documented isolation, molecular identification of etiologies and therapeutic management of affected cases. A total of 114 dogs were screened for dermatophytosis from July 2021 to January 2022 from Teaching Veterinary Clinical Complex, Pookode, Wayanad. Dermatophytes were observed in 30 (26.32 per cent) of cases presented. Chicago sky blue staining of skin scrapings and culture showed better visualization of the fungal etiology. Culturing in DTM aided in early and easy detection of dermatophytes. Dermatophytosis was most commonly encountered in puppies of less than one year of age (46.67 per cent) than adult dogs. German Shepherd breeds of dog (26.67 per cent) were more affected with dermatophytosis than other breeds.76.67 per cent of dogs were bathed in an interval of one week or less and irregularly groomed animals constituted 66.67 per cent of cases. Alopecia, pruritus, scales, papules and patches were the predominant clinical signs observed in dogs withdermatophytosis. Molecular characterization of dermatophyte isolates was carried out by using ITS gene and species was identified by RFLP using restriction enzyme Mva I. Based on the analysis of RFLP pattern, 14 isolates of M. canis, eight isolates of T. rubrum, six isolates of M. gypseum and two isolates of E. floccosum were confirmed. All the 30 dogs with dermatophytosis were grouped into three and topical therapy with shampoo containing two per cent miconazole and two per cent chlorhexidine, shampoo containing four per cent chlorhexidine and lime sulphur dip were used to treat 10 cases each. The efficacy of different treatment was evaluated based on disappearance of lesions and clinical signs, resurgence of hair and negative fungal cultures. All dogs responded to different treatments of dermatophytosis and it was found that once in three days topical application of shampoo containing four per cent chlorhexidine for average 99 days would result in faster clinical and mycological cure