IN VITRO MUTAGENESIS RESISTANCE TO ALTERNARIA BLIGHT IN CUMIN (CUMINUM CYMINUM L.)
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Date
2020
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CENTRE FOR ADVANCED RESEARCH IN PLANT TISSUE CULTURE DEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY ANAND AGRICULTURAL UNIVERSITY ANAND
Abstract
The present study entitled “In vitro mutagenesis resistance to Alternaria blight in cumin (Cuminum cyminum L.)’’ was conducted at Centre for Advanced Research in Plant Tissue Culture. AAU, Anand, Gujarat, during 2019-20. The seeds of three cumin genotypes Gujarat cumin-2 (GC-2), Gujarat cumin-4 (GC-4) and Sanand cumin-1 (SC-1) were selected for the study. The experiments were conducted for genotype specific responses for callusing and regeneration. The second experiment was conducted to standardize the in vitro mutagenesis and to isolate putative mutants. The third experiment was conducted to isolate the resistant cell lines through in vitro selection against Alternaria blight. These mutants were identified based on morphological parameters and they were subjected to molecular analysis using RAPD marker (Random Amplified Polymorphic DNA) for the detection of polymorphism among the mutant callus thus identifying the true mutants. This resistant callus was subjected to subculturing for in vitro regeneration of resistant shoots.