CHROMOSOME CHARACTERISATION OF MALABARI GOATS AND ITS CROSSES IN KERALA

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Date
1992-03-10
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES Mannuthy - Thrissur
Abstract
The chromosome prolile ot qoats v.'as bruored wiuh o vie to find its number, to prepare the karyotypes of female and male goats of the Malabari and its crosses with Saanen and Alpine and to assess its morphom-etr ic values. The experiments were carried out in jugular blood lymphocytes. The cultures were prepared using RPMl 1640 as the rr>edium. The comparative efficacy or different m.xtogens viz. PHA, PWM and their combination, with different incubation time, revealed that the combination of PHA and PWM at an incubation period of 70.5 h yielded the optimum results The diploid number of chromosomes in all the genetic groups of goats were found to be 60. Autosomes as well as X chromosomes were found to be acrooentric. The X chromosome was quite prominent ii nrn bei.-.Ofg- rlop iQnnodir of all. The Y chromosome was the shortest and the only metacontric in the complement. In karyotypes of the three genetic groups, it was observed that twenty-nine homologous pairb of autosome formed a closely graded serlarion. Sexuai dimorphism v,/as . . , .o., ,-,f y ^r-.d Y chromosomes in exhibitcG wiLn an uiiA-^;uai -- nf Y e'">-orr,r,c:r,mr-s in the females, males anu an equal t^a . .. or .. The relative length was estimated for each pair or chromosomes in percentage. The X chromosome contributed to more than five per cent of the total complement length in all the three genetic groups. In Malabari, Saanen x Malabari and Alpine x Malabari, the relative length of the Y chromosomes were 1.552+0.10, 1.321+0.09 and 1.548+0.10 respectively. The analysis of variance of relative length of X chromosomes revealed a significant difference among the three genetic groups.
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