ROLE OF ANTIOXIDANT- AND LEA GENES IN WHEAT (Triticum aestivum L.) SEEDLINGS UNDER DIFFERENT ABIOTIC STRESSES
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Date
2011
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PAU Ludhiana
Abstract
Two wheat cultivars, C306 (drought tolerant) and PBW343 (drought susceptible) were
compared for their response to water stress (WS), salt stress (ST) and combined stress (WS
plus ST) during germination. Their response was studied in the form of growth and
antioxidant potential in roots and shoots and in the form of 11 LEA (group 2, group 3 and
group 4) genes’ expression in shoots. Drought tolerant cultivar, C306 was found to be more
salt sensitive in comparison to drought sensitive cultivar PBW 343. Salt sensitivity of C306
was related to more MDA contents, lower levels of antioxidant enzymes, lesser proline
contents in roots, lesser dry weight accumulation under ST than the levels observed under
WS. Salt-insensitivity of PBW343 was related to increased levels of H2O2 followed by
increased ascorbate and ascorbate/dehydroascorbate ratio under ST whereas such features
were almost lacking under WS. Dry matter accumulation under ST was also more than under
WS in PBW343. Above mentioned responses under combined stress were more similar to WS
than ST in C306 and were more similar to ST than to WS in PBW343. Among 11 LEA genes,
nine genes were induced by higher amount under WS than under ST in C306. This also
favored salt-susceptibility of C306. In PBW343, group 2 and group 4 genes were almost
equally induced under WS and ST but group 3 genes were more induced under ST than WS.
Two LEA genes, Wdhn13 and Td25a were more salt-responsive as these were induced more
under ST than WS in both cultivars.
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