CHARACTERISATION OF MYOSTATIN GENE IN DIFFERENT BREEDS OF RABBITS”
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Date
2010
Authors
Journal Title
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Publisher
Anand Agricultural University, Anand
Abstract
Myostatin is a member of the Transforming Growth Factor (TGF)- β
superfamily and highly conserved in gene structure among vertebrate species.
Myostatin (nick name - Schwarzenegger gene) is a strong negative regulator of
skeletal muscle growth. Myostatin is a cytokine and is expressed at high level in
skeletal muscles.
DNA extraction was carried out from the blood collected from five animals of
each breed (Soviet chinchilla, White giant & Desi) of rabbit (Oryctolagus cuniculus)
by phenol : chloroform method. Locus specific primers were used to amplify gene
fragments of MSTN exon I (499 bp), MSTN exon II (570 bp) and MSTN exon III
(523 bp) in the three breeds of rabbit (Oryctolagus cuniculus).
Description
Keywords
Animal Biotechnology, Study