Studies on in vitro propagation of promising grape (Vitis Vinifera L.) rootstocks
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Date
2014
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Publisher
Punjab Agricultural University, Ludhiana
Abstract
The present investigation entitled “Studies on in vitro propagation of promising grape (Vitis
Vinifera L.) rootstocks” was carried out in the Tissue Culture Laboratory of the Department
of Fruit Science, Punjab Agricultural University, Ludhiana during 2013-2014. The MS
medium fortified with BAP (1.0 mg/l) + Kinetin (1.0 mg/l) shows maximum establishment
45.54 % and 40.80 % from nodal segments and shoot tips respectively. Rootstock R 110 and
1613 C rootstock exhibited the maximum nodal segment and shoot tip establishment 47.37 %
and 35.42 % respectively. The maximum shoot regeneration (65.67 %) was recorded in MS
medium supplemented with BAP (1.0 mg/l) and Kinetin (1.0 mg/l). Degrasset rootstock gave
the maximum (52.89 %) shoot regeneration. Maximum number of shoots (3.63) per explant
were produced at BAP (1.5 mg/l). Maximum shoot length (3.13 cm) was recorded on MS
supplemented with BAP (0.5 mg/l) and Kinetin (0.5 mg/l) medium. The maximum shoot
length (1.73 cm) was recorded in 1613 C rootstock. Maximum rooting was (64.95 %)
recorded with MS media fortified with IBA (0.1 mg/l) and NAA (0.1 mg/l). Maximum
rooting (55.64 %) was recorded in Degrasset rootstock. Maximum number of roots (13.75)
was recorded in R 110 rootstock on IBA (0.01 mg/l) and NAA (0.01 mg/l). Maximum length
of roots (5.66 cm) was recorded in rootstock Degrasset cultured on MS medium
supplemented with IBA (0.1 mg/l). The 2:1:1 ratio of peat perlite and vermiculite and
Degrasset rootstock is the best rootstock for plantlet establishment under ex vitro conditions.
Description
Keywords
Fruit Science, Vitis Vinifera L., Biotechnology, rootstocks, grape, Kinetin