Evaluation of different osmo-protectants against water stress in Bt cotton (Gossypium hirsutum L.)

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Date
2020
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Punjab Agricultural University, Ludhiana
Abstract
The investigation entitled “Evaluation of different osmo-protectants against water stress in Bt cotton (Gossypium hirsutum L.)” has been carried out at Punjab Agricultural University, Regional Research Station, Bathinda and Dr. J.C. Bakhshi Regional Research Station, Abohar during kharif 2018. The experiment has been laid out in split plot design with three replications. Main plots consisted of three levels of irrigation i.e. control (irrigation as per recommendation), one irrigation at 5 weeks after sowing (WAS) and no irrigation. Sub plots consisted of eight osmoprotectants (application started from 70-80 days after sowing) i.e. control (water spray), 2 % urea (4 sprays at 7 days interval), 2% KNO3 (4 sprays at 7 days interval), 1% thiourea (single spray), salicylic acid @ 50 ppm (SA50) single spray, salicylic acid @ 100 ppm (SA100) single spray, glycine betaine @ 100 ppm (GB100) single spray, pink pigmented facultative methylotrops (PPFM) @ 1% three sprays at 10 days interval. Experiment results revealed that crop with recommended irrigation attained the maximum plant height and higher dry matter than irrigation at 5 WAS and no irrigation. CGR, RGR and NAR were significantly affected by irrigation treatments. Higher number of monopods, sympods and main stem internodes were obtained with control among the irrigation treatments. Phenological stages were also longer under recommended irrigation. Total bolls plant-1, opened bolls plant-1 and boll weight were significantly higher under recommended irrigation at both the locations. All these resulted in significantly higher seed cotton yield by margin of 26.0 and 24.6% under recommended irrigation as compared to no irrigation at Bathinda and Abohar, respectively. Quality parameters like ginning outturn (GOT), lint index, 2.5% fibre span length and fibre strength were deteriorated significantly under no irrigation as compared to recommended irrigation. Higher nutrient content of stover, seed and lint was attained by control. Among the osmoprotectants, application of 2% KNO3 resulted in maximum plant height, dry matter accumulation, sympods and main stem internodes plant-1 and also enhanced the yield attributes as indicated by significantly increased seed cotton yield (i.e. 3436 and 3286 kg ha-1 at Bathinda and Abohar, respectively) over control (i.e. 2591 and 2351 kg ha-1 at Bathinda and Abohar, respectively) followed by GB100, SA100 and SA50 as compared to water spray (control). While, 1% thiourea produced the lowest seed cotton yield at both the locations. Quality parameters like GOT, lint index, 2.5% fibre span length and fibre strength, nutrient content of stover, seed and lint and irrigation water use efficiency were increased with application of 2% KNO3, salicylic acid and glycine betaine.
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