Studies on Rhizogenesis of some genotypes of Rose through stem cuttings

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Date
2011
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SKUAST-K
Abstract
Rose is the best known and most popular of all garden flowers throughout the world. It is generally propagated by stem cuttings or budding desirable cultivars on rose rootstocks. Application of growth regulator treatments to the cuttings has been found very effective in improving the rooting characteristics in roses. Present research project entitled “Studies on rhizogenesis of some genotypes of rose through stem cuttings” was carried out in the field at the Research Farm, Division of Floriculture, Medicinal and Aromatic Plants, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar during 2010 for improving rooting characteristics. The experiment was laid out in factorial completely randomized design replicated thrice (ten cuttings/replication). Four experiments were conducted to evaluate the performance of various levels of growth regulators (0, IBA 500, 1000, 1500, NAA 500, 1000, 1500 ppm) and different growing medium (Sand, sand 85% + manure 15%, sand 70% + manure 30%, sand 70% + manure 15% + soil 15%, sand 85% + soil 15%) on the rooting characteristics of some commercial cut flower cultivars and rootstocks of rose. First experiment was conducted to evaluate the effect of growth regulator on the rooting of three rootstocks of rose. Growth regulator and genotype had a significant influence on all rooting parameters except callusing. Application of IBA at 1000 ppm recorded minimum days to root initiation (23.33), maximum rooting (72.22%), root length (6.42 cm) and field survival (82.38%). Maximum primary root number (8.59) was observed with NAA (1000 ppm). NAA was found more effective than IBA for Rosa indica. Among the genotype, Rosa indica performed best recording maximum rooting (75.24%), primary root number (9.48), root length (6.40 cm), new leaf growth on cuttings (59.05%) and field survival (96.98%) followed by Rosa banksiae. Performance of Rosa bourboniana was poor recording lowest values for all these parameters. Second experiment was designed to study the rooting characteristics of four commercial cut flower rose cultivars as influenced by application of growth regulator. Rooting characteristics were significantly improved by auxin treatments. IBA at 1500 ppm recorded minimum days to root initiation (22.55), maximum rooting (76.67%), primary root number (12.57), root length (5.87 cm) and field survival (73.13%). IBA at 1000 ppm recorded maximum new leaf growth in cuttings (14.17%). Among the genotypes, First Red proved superior to other cultivars with respect to majority of rooting parameters recording maximum rooting (66.67%), primary root number (11.29), root length (4.98 cm) and field survival (76.28%). Minimum days to root initiation (21.61) and maximum new leaf growth on cuttings (13.81%) was observed in Noblesse. There was non-significant effect of growth regulator on callusing percentage. In the third experiment, significant influence of growing medium was observed on the rooting characteristics of rose rootstocks. M3 medium (sand 70%+ manure 30%) recorded minimum days to root initiation (22.13), maximum rooting (83.33%), primary root number (15.52), root length (8.88 cm), new leaf growth (51.11%) and field survival (85.46%). Second best medium was M2 (sand 85%+ manure 15%) while M5 medium (sand 70%+soil 15%+ manure 15%) gave very poor performance. Among the genotypes, Rosa indica gave better results recording minimum days to root initiation (22.70), maximum rooting (90.00%), primary root number (21.21), root length (10.10 cm), new leaf growth (47.33%) and field survival (97.78%), while Rosa bourboniana recorded minimum values for all the parameters with maximum days to root initiation (25.02). There was non-significant effect of growing medium on callusing percentage. In the fourth experiment, significant influence of growing media was observed on the improvement of rooting in all the four commercial cut flower cultivars of rose. Growing medium M3 (sand 70% + manure 30%) recorded maximum callusing (95.00%), rooting (72.50%), primary root number (8.26), root length (4.62 cm), new leaf growth (33.18%) and field survival (74.91%). Second best medium was M2 (sand 85% + manure 15%) while M5 medium (sand 70% + soil 15% + manure 15%) yielded poor results. M1 (sand 100%) took minimum days (23.12) to root initiation. Among the genotypes, Naranga recorded maximum rooting (70.00%), primary root number (7.38) and new leaf growth (32.71%). First Red recorded maximum callusing (92.00%), root length (3.91 cm) and field survival (77.06%) while Noblesse took minimum number of days (21.92) to root initiation.
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M.Sc Thesis submitted to SKUAST Kashmir
Keywords
Growing medium, IBA,, NAA, rootstocks, FLORICULTURE AND LANDSCAPING ARCHITECTURE
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