CHARACTERIZATION OF CAMTA TRANSCRIPTION FACTOR FAMILY WITH RESPECT TO DROUGHT TOLERANCE IN CHICKPEA (Cicer arietinum L.)

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dc.contributor.advisorBHUTIA, K. L.
dc.contributor.authorKUNDU, SAYANTA
dc.date.accessioned2023-07-06T10:34:38Z
dc.date.available2023-07-06T10:34:38Z
dc.date.issued2021
dc.description.abstractChickpea is the second most valued, protein rich annual legume that is abundant in dietary fibers, minerals, iron and phosphorus. It is cultivated in almost 50 countries and India is the largest producer of chickpea. However, the production is not enough to fulfill the demand. As chickpea is grown in arid and semi-arid region, the production faces huge loss under drought stress. So, the only solution of this problem is to generate drought tolerant varieties. CAMTA is a transcription factor family which specifically binds to Ca2+-calmodulin complex and regulates gene expression in response to various stress conditions including drought. Therefore, CAMTA family of chickpea was characterized using in silico and qRT-PCR based wet lab approaches under drought stress. Fifteen protein members basically encoded by 7 genes and their splice variants of CAMTA family were identified in chickpea and new names were assigned (CaCAMTA) to them as it is the first genome wide characterization of chickpea CAMTA gene family. The collected protein annotation and biological function depicts that the genes code for calmodulin-binding transcription activator proteins of different isoforms having role in gene regulation in response to stress tolerance i.e., freezing, salt and wounding etc. Among numerous cis-acting elements identified from 5’ upstream of CaCAMTA genes, MYBCORE, ABRELATERD1 and MYB1AT are the cis-acting elements having important roles during water stress conditions. The conserved domains present on polypeptide chains of each member of CaCAMTA family are CG-1 and IPT domain, ankyrin containing repeat domain, and IQ domain functioning as DNA binding, signal transducing and calmodulin binding domain. Dendrograms obtained for top 5 orthologs of 7 base genes suggested best hit orthologs gene were from Medicago truncatula and Vigna unguiculata. As the 15 proteins were basically the products of 7 CAMTA genes and their splice variants, products of the same CaCAMTA gene and their splice variants were clustered together in amino acid based phylogenetic analysis. Interaction analysis of CaCAMTA proteins revealed that each CaCAMTA proteins have network of interactions with various characterized and uncharacterized proteins. Under 15 days of drought treatment, growth and development of three chickpea genotypes namely RSG 888, Vijay and Pusa 256 were not much hampered, therefore were considered as drought tolerant genotypes. Similarly, the growth of chickpea genotype JG 14 was severely reduced during 15 days of drought treatment as compare to control plant, therefore, it was considered as susceptible genotype. The chlorophyll contents in treated plants were increased in RSG 888 and Vijay, but decreased in Pusa 256 and JG-14. The differential expression study of CAMTA genes using qRT PCR in chickpea under drought stress showed that CaCAMTA 1.1.2, 6.2.3, 7.1.1, and 5.1.1 are upregulated among the tolerant genotypes i.e., RSG 888, Vijay, and Pusa 256; and CaCAMTA 1.2.1 is downregulated in tolerant genotypes RSG 888 and Pusa 256 and up-regulated in susceptible genotype JG-14. The genome wide characterization of CAMTA family in chickpea will broaden the idea of drought stress mechanisms in Chickpea. The candidate gene-based markers could be designed targeting these differentially expressed genes to be utilized in marker trait association studies and if found significantly associated to yield attributing traits of chickpea, it can be utilized in marker assisted breeding of Chickpea for drought tolerance.en_US
dc.identifier.otherM/AB/454/2019-20
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810198001
dc.keywordsDrought, Chickpea, CaCAMTA, in silico, qRT PCR, Calmodulin.en_US
dc.language.isoEnglishen_US
dc.pages70+ i-xxxi (bibliography + appendices)en_US
dc.publisherDRPCAU, PUSAen_US
dc.subAgricultural Biotechnologyen_US
dc.themeCharacterization of CAMTA transcription factor family with respect to drought tolerance in chickpea (Cicer arietinum L.)en_US
dc.these.typeM.Scen_US
dc.titleCHARACTERIZATION OF CAMTA TRANSCRIPTION FACTOR FAMILY WITH RESPECT TO DROUGHT TOLERANCE IN CHICKPEA (Cicer arietinum L.)en_US
dc.typeThesisen_US
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