Lipid peroxidation and antioxidant pathway in specific leaves of Brassica juncea L. infected with Albugo candida
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Date
2008
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Publisher
CCSHAU
Abstract
The present study was undertaken to investigate the lipid peroxidation and
antioxidant pathway in specific leaves of resistant and sensitive cultivars of
Brassica juncea L. infected with Albugo candida. Following inoculation of third
leaf with Albugo candida spores higher electrolyte leakage, MDA and H2O2
content indicating enhanced lipid peroxidation was observed in sensitive cultivar
Varuna. Activity of H2O2 scavenging enzyme viz. peroxidase and catalase
increased in both the cultivars but more pronounced enhancement was found in
resistant cultivar except super oxide dismutase, as its activity was found to increase
in Varuna but declined sharply in RH781. The activity of Halliwell- Asada
pathway enzymes i.e. ascorbate peroxidase and glutathione reductase enhanced in
both the cultivars but the elevation was more in RH781. Ascorbate and reduced
glutathione level increased specifically in RH781 whereas, oxidized glutathione
was found to be higher in Varuna signifying more oxidative damage.
The study shows noticeable enhancement in lipid peroxidation, activities of
enzymes of antioxidant pathway and ascorbate and glutathione content in third leaf
on 3 DAI. Thereafter, the increase was more in second leaf as compared to fourth
leaf in both the varieties indicating that transduction of pathogen attack signal
might be towards the upper leaves via phloem than the lower leaves.
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Keywords
Planting, Pigeonpeas, Crossing over, Rapd, Genotypes, Genetics, Dna, Polymorphism, Diseases, Developmental stages