SUB-ACUTE TOXICITY OF INDOXACARB AND ITS REVERSAL WITH HESPERIDIN AND ASCORBIC ACID IN RATS
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Date
2023-03-23
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COLLEGE OF VETERINARY AND ANIMAL SCIENCS, MANNUTHY, KERALA VETERINARY AND ANIMAL SCIENCES UNIVERSITY
Abstract
The present study was conducted to evaluate the sub-acute toxicity of indoxacarb and its reversal with hesperidin and ascorbic acid in rats. Female Wistar albino rats were divided into five groups of six animals each. Group I received 1 per cent carboxy methyl cellulose (CMC) as vehicle control and group II received indoxacarb (IND) at the dose of 30 mg/kg body weight in 1 per cent CMC. Group III and IV received hesperidin (HSP) and ascorbic acid (ASC) at 100 and 50 mg/kg body weight dose levels respectively while Group V received hesperidin and ascorbic acid together at the above doses, along with indoxacarb in 1 per cent CMC. The vehicle/test substances were administered orally for 28 days on daily basis. During the treatment period the daily body weight gain, feed and water intake were measured along with the weekly assessment of hepatic and renal function through serum biomarkers. After 28 days of treatment, the animals were euthanized and organs were collected. The mean weekly weight gain and feed intake of the indoxacarb alone treated group was lower than other groups during the entire study period while a significant reduction in mean weekly water intake was observed in this group from weeks 2 to 4. The treatment groups III, IV and V with HSP, ASC and a combination of both significantly rectified the above parameters. The indoxacarb induced anomalous levels of serum biomarkers (ALT, AST, creatinine and BUN) and the reduction in the weight of the liver were significantly restored after treatment with HSP, ASC and their combination. The variation in the antioxidant parameters, catalase and superoxide dismutase, in brain, heart, liver, spleen, kidney, lungs and reproductive organs were also rectified by the treatment with the above agents. The histological examination of the organs revealed hepatotoxicity and nephrotoxicity in indoxacarb alone group, whereas it was reversed in treatment groups.From the present study, it can be concluded that both HSP and ASC can reverse the toxicity of indoxacarb in rats where the combination of these agents offers the most effective management of sub-acute toxicity. However, studies with further synergistic doses of HSP and ASC can be conducted for selecting the appropriate treatment regimen