BIOCHEMICAL AND MOLECULAR ANALYSIS OF WHEAT INVERTASE
Loading...
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The present investigation was aimed to study the interactive effect of high temperature (HT) and thiourea (TU) on various morpho-physiological and biochemical parameters in four wheat (Triticum aestivum L.) cultivars viz. C 306, C 273, PBW 343 and PBW 534. High temperature caused reduction in root/shoot length and dry weight while application of TU was effective in improving both root/shoot lengths and dry weight. High temperature caused increase in invertase activities in both root and shoot in correspondence with an increase in total sugars, reducing sugars and decrease in non reducing sugars. Thiourea alleviated HT response by further elevating invertase activities which lead to higher seedling dry mass (soluble sugars and proteins). Inhibitory effect of iodine and Hg+2 on invertases confirmed the presence of –SH group at the active catalytic site of enzyme. The differential response to metal ions suggested distinctive nature of invertases in four cultivars. High temperature also disrupted membrane stability parameters by increasing TBARs content and membrane permeability in root/shoot whereas TU application ameliorated this effect by reducing TBARs content and membrane injury in wheat seedlings. Sequence analysis of wall bound invertase was done using forward and reverse primers based on nucleotide sequence of Ivr1 gene. For this genomic DNA was extracted, amplified and sequenced. Polymorphism such as SNPs (single nucleotide polymorphism) and indels (insertions or deletions) were worked out and primers redesigned based on sequence polymorphism. Redesigned primers showed very slight expected dominant polymorphism in the studied cultivars due to A, B and D genomes of wheat