CLINICO-EPIDEMIOLOGICAL STUDIES AND MOLECULAR DIAGNOSIS OF FELINE PANLEUKOPENIA IN CATS

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dc.contributor.advisorR. L, Rathish
dc.contributor.authorBAKDE, RIYA ASHOK
dc.date.accessioned2021-01-12T07:11:42Z
dc.date.available2021-01-12T07:11:42Z
dc.date.issued2019-10-21
dc.description.abstractThe present study envisaged to undertake serosurveillance and to investigate the clinico-epidemiological features of feline panleukopenia in North Kerala. The study also aimed at comparing haemagglutination inhibition test (HI) and Loop Mediated Isothermal Amplification (LAMP) with Polymerase chain reaction (PCR) for detection of Feline Panleukopenia Virus (FPV) from faecal samples of affected cats. An indirect sandwich ELISA was done on 79 unvaccinated healthy cats to detect circulating antibodies against FPV, which revealed a prevalence of 29.11 per cent. Analysis across various epidemiological profiles revealed no statistical difference, indicating equal rate of exposure across all epidemiological parameters. Feline panleukopenia virus DNA was detected in 34 out of 40 faecal samples by PCR. Epidemiological analysis revealed higher occurrence of feline panleukopenia in cats aged less than six months, among females and non-descript cats. Stray cats, multiple cat households and cats without any deworming history were more frequently affected. Pyrexia, dysentery, vomiting, dehydration and respiratory distress were predominant clinical findings. The affected cats suffered from anaemia, thrombocytopenia, leucopoenia and elevated liver enzymes. Ultrasonography revealed gas and fluid filled intestinal loops. PCR targeting the VP2 gene of FPV resulted in 698bp amplicons which was sequenced. BLAST analysis of the sequence revealed similarity to feline panleukopenia virus. Haemagglutination was observed in 25 faecal samples, out of which haemagglutination inhibition assay confirmed the presence of FPV in ten samples and a kappa score of 0.1 indicated poor agreement to PCR. In contrast, LAMP detected 36 out of 40 samples tested by PCR. Even though all the PCR negative samples were lamp negative, two of the PCR negative samples were positive by LAMP. Statistical analysis revealed a high quotient of agreement between PCR and LAMP. LAMP had 100 per cent sensitivity and positive predictive value with 67 per cent specificity and 94 per cent negative predictive value compared to PCR. The potential of LAMP as a diagnostic tool in limited resource settings can be exploited for rapid diagnosis of infectious diseases.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810159878
dc.keywordsfeline panleukopenia, HI,LAMP, FPVen_US
dc.language.isoEnglishen_US
dc.pages105en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANADen_US
dc.research.problemFELINE PANLEUKOPENIA IN CATSen_US
dc.subVeterinary Epidemiology and Preventive Medicineen_US
dc.themeCLINICO-EPIDEMIOLOGICAL STUDIES AND MOLECULAR DIAGNOSIS OF FELINE PANLEUKOPENIA IN CATSen_US
dc.these.typeM.V.Sc.en_US
dc.titleCLINICO-EPIDEMIOLOGICAL STUDIES AND MOLECULAR DIAGNOSIS OF FELINE PANLEUKOPENIA IN CATSen_US
dc.typeThesisen_US
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