STUDY ON PREVALENCE OF TUBERCULOSIS IN HUMAN AND ANIMAL POPULATION WITH SPECIAL REFERENCE TO ITS ZOONOTIC SIGNIFICANCE

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dc.contributor.advisorBrahmbhatt, M. N.
dc.contributor.authorPARMAR, BHUPENDRA C.
dc.date.accessioned2018-04-27T08:16:41Z
dc.date.available2018-04-27T08:16:41Z
dc.date.issued2010
dc.description.abstractThe aim of the present study was to isolate, identify and characterise Mycobacteria from various clinical specimens of human beings, animals and from environment. Microbiological examination of 600 samples (150 from human, 400 from animals and 50 from environment) was carried out to study the prevalence of Mycobacteria. Mycobacterium bovis and Mycobacterium tuberculosis were isolated during the study period from various specimens of animals and human, viz. milk 18 (out of 148) and nose swabs 40 ( out of 252) from cattle; throat swab 1 (out of 24), nose swab 6 (out of 62) and sputum 6 (out of 64) samples from human. However, no non- tuberculous Mycobacteria {Mycobacterium forttiitum) were isolated from soil and water during the study period. All these clinical isolates of Mycobacteria were subjected to Z-N staining. Biochemical tests, viz. Catalase test. Niacin detection. Nitrate reduction, Pyrazinamidase activity and T2CH (Thiophene- 2- carboxyllic acid hydrazide) and PCR (Polymerase chain reaction). Single intradermal test (tuberculin test) was carried out in 260 animals of LRS and HF farm. Among these, 42 cattle were found positive for tuberculin test. From 42 tuberculin positive and 218 tuberculin negative cattle, 37 and 21 isolates, respectively, of Mycobacterium bovis were recovered. Single intradermal test (Mantoux test) was carried out on 50 human beings, none was found positive for Tuberculosis; eventhough 13 isolates oiMycobacterium tuberculosis were recovered. The results of this investigation indicated that the frequency of occurrence of organism was higher in the cattle (81.69 %) than the human (18.31 %); frequency of occurrence was higher in exotic cattle (37.29 %) than indigenous cattle (1.41 %). In man, the frequency of occurrence of organism was higher in the males (11.11 %) than the females (0 %). Among cattle; females are more susceptible than males. Among the various clinical specimens collected, 64.80 per cent isolates of Mycobacteria were from nose swab, followed by milk (25.50 %), sputum (8.50 %) and throat swab (1.20 %). Enviroimiental screening of the soil and water yielded zero isolate of Mycobacteria. It was concluded that, the overall prevalence of tuberculosis was higher in animals as compared to human and environment. Exotic cattle are more susceptible to tuberculosis than indigenous cattle. Single intradermal test (tuberculin test) is useful for the detection of primary infection of tuberculosis, but it is not important in human for detection of tuberculosis. Biochemical tests, viz. Catalase test. Niacin detection. Nitrate reduction, Pyrazinamidase activity and T2CH (Thiophene 2 carboxyllic acid hydrazide) are effective for differentiation between Mycobacterium tuberculosis and Mycobacterium bovis. All the isolates of Mycobacteria were screened for PCR for the presence of genes viz. p34 for Mycobacterium tuberculosis complex and hupB for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis using specific primers. Being a less expensive and easily available mediimi, LJ medivmi with or without sodium pyruvate can be recommended for the routine microbiological work in the microbiology and public health laboratories for the study of prevalence of tuberculosis in human and animal population.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810044689
dc.keywordsSTUDY ON PREVALENCE OF TUBERCULOSIS, HUMAN AND ANIMAL POPULATION, WITH SPECIAL REFERENCE TO ITS ZOONOTIC SIGNIFICANCEen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.research.problemSTUDY ON PREVALENCE OF TUBERCULOSIS IN HUMAN AND ANIMAL POPULATION WITH SPECIAL REFERENCE TO ITS ZOONOTIC SIGNIFICANCEen_US
dc.subVeterinary Public Healthen_US
dc.subjectVETERINARY PUBLIC HEALTHen_US
dc.subjectA STUDYen_US
dc.themeSTUDY ON PREVALENCE OF TUBERCULOSIS IN HUMAN AND ANIMAL POPULATION WITH SPECIAL REFERENCE TO ITS ZOONOTIC SIGNIFICANCEen_US
dc.these.typePh.Den_US
dc.titleSTUDY ON PREVALENCE OF TUBERCULOSIS IN HUMAN AND ANIMAL POPULATION WITH SPECIAL REFERENCE TO ITS ZOONOTIC SIGNIFICANCEen_US
dc.typeThesisen_US
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