EFFECT OF MEDIA ON IN-VITRO MATURATION OF BOVINE FOLLICULAR OOCYTES
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Date
2016-07
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Assam Agricultural University, Khanapara, Guwahati
Abstract
The present research was planned to study the effect of different concentrations of Vitamin E and Oestrous cow serum and their combination on in-vitro maturation of bovine follicular oocytes incubated in TCM-199 medium containing Follicular fluid, Oestradiol-17β, p-FSH, Gentamicin, Sodium Pyruvate, Cysteamine and Fetal Bovine Serum at 38.5 0C with 5% CO2 for 24 hours in a CO2 incubator. A total of 825 oocytes were recovered from slaughterhouse ovaries of cattle by aspiration cum slicing technique and graded as A, B, C and D with the recovery rates of 77.64 ± 0.02, 11.60 ± 0.01, 6.84 ± 0.01 and 3.91 ± 0.01 per cent, respectively. A total of 704 oocytes of grade A and B were used in the study. Maturation of oocytes was determined on the basis of rate of cumulus cell expansion and polar body formation.
Vitamin E concentrations at 0, 100 and 150 µM levels in the maturation medium resulted in cumulus cell expansion rate of 74.46 ± 2.68, 74.28 ± 1.66 and 73.59 ± 0.78 per cent, respectively, while polar body formation rates were found to be 44.02 ± 4.37, 50.06 ± 2.40 and 45.43 ± 4.20 per cent, respectively. Oocytes maturation rates both in terms of cumulus cell expansion and polar body formation were not affected by levels of Vitamin E supplementation in the in-vitro maturation medium. Oestrous Cow Serum supplementation at 0, 20 and 30 per cent levels to the in-vitro maturation medium resulted in the cumulus cell expansion rate of 74.46 ± 2.68, 68.41 ± 2.40 and 63.90 ± 3.49 per cent, respectively; corresponding figures for polar body formation rate were 44.02 ± 4.37, 32.10 ± 2.84 and 29.50 ± 0.67 per cent. Addition of either 20 or 30 per cent Oestrous Cow Serum in the maturation medium did not improve the oocytes maturation rate both in terms of cumulus cell expansion as well as polar body formation. Cumulus cell expansion and polar body formation rates were recorded as 67.20 ± 3.45 and 38.26 ± 2.28 per cent, respectively when 100 µM Vitamin E and 20 per cent Oestrous cow serum combination was used in the in-vitro maturation medium. The corresponding rates for the control medium (without supplementation) were 74.46 ± 2.68 and 44.02 ± 4.37 per cent.
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