Cytological studies of mutagen treated seeds of karanj (Pongamia pinnata L.)
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Date
2009
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Publisher
CCSHAU
Abstract
The studies involve the treatment of Pongmia pinnata L. Pierre (Karanj) with two mutagens
i.e. gamma rays and Ethyl methane sulphonate (EMS) at different concentrations. Three hundred seeds
of Karanj were treated for each dose of gamma rays at the dose rate of 10 kR, 20 kR, 30 kR, 40 kR, 50
kR, 60 kR and 70 kR. Similarly three hundred seeds of Karanj were soaked in freshly prepared solution
of EMS for each concentration of 0.1%, 0.2%, 0.3%, 0.4%, 0.5% and 0.6% for 6 hours. The treated
seeds were sown in the nursery of the Department of Forestry, CCS HAU, Hisar in three replications.
The results indicated the effect of both mutagens on the parameters studied. The percent germination
decreased as the dose increased in case of both the mutagens. The treatments were significantly differ
from each other in case of percent seed germination. LD50 value was found to be 20 kR in case of
gamma rays and 0.3% in case of EMS treatment. Root length and plant height decreased with the
increase in concentration in case of both the mutagens. The treatments were significantly differ from
each other for root length in case of EMS treatment. Collar diameter showed different pattern for both
the mutagens. Treatments differ significantly for collar diameter in case of gamma rays and collar
diameter decreased as the dose increase, but in case of EMS, the collar diameter increased as
concentration increase but at 0.4% show a slight decrease. There was no significant difference among
treatments for number of leaves per plant in case of both the mutagens. Mitotic and metaphase indices
show an overall decrease with increase in concentration in both the mutagens. The chromosomal
abnormalities were increased with an increase in concentrations in case of both the mutagens. Anaphase
bridges and distorted metaphase were the main abnormalities found in case of gamma rays and
anaphase laggard, anaphase bridge and distorted metaphase were the main abnormalities found in case
of EMS treatment. The use of mutagens have been suggested with precautionary measures.
Description
Keywords
Karanj, Pongamia pinnata L., Mutagens, Gamma rays, Ethyl methane sulphonate, Chromosomal aberrations