TOXICOPATHOLOGICAL AND GENOTOXICITY STUDIES OF SODIUM DICHROMATE IN WISTAR RATS
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Date
2008
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Anand Agricultural University
Abstract
The present research work was conducted on 30 male and 30 female wistar
rats to study the toxicopathological and genotoxic effects of repeated dose (28 days)
of sodium dichromate. Wistar rats were randomly divided into 6 different groups with
five males and five females in each group. Animals of group I to IV were given
0.625mg/kg b.wt, 1.25 mg/kg b.wt, 2.5 mg/kg b.wt, 5 mg/kg b.wt sodium dichromate
by gavage for 28 days whereas group V and group VI were administrated only water
as negative (vehicle) control and cyclophosphamide as positive control respectively.
After completion of 28 days treatment, blood samples were collected for haematology
and serum biochemical analysis from retro-orbital plexus with the help of capillary
tube. The animals were sacrificed by cervical dislocation and bone marrow was
collected for genotoxicity study. Necropsy examination was performed in all
sacrificed animals and gross lesions were recorded. Tissue samples (lung, liver,
kidney, spleen, intestine, testes, brain and stomach) were collected in 10% formalin
solution for histopathological examination.
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Keywords
Veterinary Pathology, Study