SEROLOGICAL DETECTION OF TOXOCARA CANIS INFECTION IN DOGS OF KERALA USING RECOMBINANT CATHEPSIN-L1 AND TOXOCARA EXCRETORY SECRETORY
| dc.contributor.advisor | Varghese, Anju | |
| dc.contributor.author | A, NANDINI | |
| dc.date.accessioned | 2021-01-05T08:04:13Z | |
| dc.date.available | 2021-01-05T08:04:13Z | |
| dc.date.issued | 2019-08-19 | |
| dc.description.abstract | Present study was conducted on the expression of two important proteins of Toxocara canis viz. cathepsin-L1 and TES-26 and to evaluate the diagnostic potential of these two recombinant antigens in canine toxocarosis. Adult T. canis worms were collected from puppies and total RNA was isolated and cDNA was synthesized. The coding sequences of cathepsin-L1 and TES-26 genes, respectively were amplified using specific forward and reverse primers. The PCR products obtained from cDNA got amplified at 1083 and 793 bp, respectively of cathepsin-L1 and TES-26. These proteins were subsequently expressed in Escherichia coli pPROEXHT-b expression vector. Recombinant proteins were purified using Ni-NTA chromatography. Recombinant cathepsin-L1 and TES-26 proteins were evaluated for their potential in the sero-detection of T. canis infection in both owned and stray dogs by IgG ELISA. Sera collected from 157 owned dogs were screened for anti-T. canis IgG antibodies with recombinant cathepsin-L1 and TES-26 antigen. ELISA with recombinant cathepsin-L1 showed sero-reactivity of 39/157 (24.8 per cent) in owned dogs and stray dogs showed 18/40 (45 per cent). Likewise, ELISA with recombinant TES-26 gave seroreactivity with 78/157 (49.7 per cent) owned dogs. When the stray dogs were screened with the same antigen showed a positive percentage of 28/40 (70 per cent) was recorded. As a result of this comparative study of two recombinant antigens, TES-26 gave highest positive ELISA reactivity for stray dogs followed by cathepsin-L1. rTES-26 antigen is highly sensitive in the detection of T. canis infection in owned and stray dogs compared to cathepsin-L1. Cross reactivity of these antigens were checked with dog sera which is positive for other helminths like, Ancylostoma caninum, Dirofilaria immitis, D. repens, Diphyllobothrium latum, Spirometra spp. and Babesia gibsoni. The above results showed that two recombinant antigens are cross-reacting with all the above canine parasites. The two antigens are sensitive in the detection of T. canis infection in dogs; however, the specificity of these antigens in canines needs to be further studied. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810159180 | |
| dc.keywords | TOXOCAROSIS,CATHEPSIN-L1,TES-26 ANTIGENS , | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 57 | en_US |
| dc.publisher | COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD | en_US |
| dc.research.problem | TOXOCARA CANIS INFECTION IN DOGS OF KERALA USING RECOMBINANT CATHEPSIN-L1 AND TOXOCARA EXCRETORY SECRETORY ANTIGENS | en_US |
| dc.sub | Veterinary Parasitology | en_US |
| dc.theme | SEROLOGICAL DETECTION OF TOXOCARA CANIS INFECTION IN DOGS OF KERALA USING RECOMBINANT CATHEPSIN-L1 AND TOXOCARA EXCRETORY SECRETORY ANTIGENS | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | SEROLOGICAL DETECTION OF TOXOCARA CANIS INFECTION IN DOGS OF KERALA USING RECOMBINANT CATHEPSIN-L1 AND TOXOCARA EXCRETORY SECRETORY | en_US |
| dc.type | Thesis | en_US |