Callus development, plant regeneration and in vitro selection for drought tolerant cell lines of wheat [Triticum aestivum l.]

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dc.contributor.advisorRattan Singh
dc.contributor.authorMehla, Ankur
dc.date.accessioned2016-11-22T13:42:46Z
dc.date.available2016-11-22T13:42:46Z
dc.date.issued2007
dc.description.abstractWheat (Triticum aestivum L.) is world’s leading cereal grain and also the most important food crop. In recent years a considerable improvement has been made in wheat using in vitro and in vivo techniques. The present study was planned to study the callus development and plant regeneration potential of wheat and to screen ten wheat genotypes viz., WH-147, WH-711, PBW-343, WH-542, HD-2687, WH-533, PBW-502, WH-1025, WH-773 and C-306 for drought tolerance using in vitro and in vivo techniques. The mature embryos of five wheat genotypes (WH-147, WH-711, PBW-343, WH-542 and WH-533) were cultured on MS1 (MS + 2.0% 2,4-D), MS2 (MS + 2.5% 2,4-D) and MS3 (MS + 3.0% 2,4-D) media for callus induction and regeneration. The calli were screened for drought tolerance by culturing them on MS2 media supplemented with various concentrations of PEG (6000 MW) viz., 7.5%, 10.0%, 12.5%, 15.0% and 17.5%. For in vivo drought screening, the seeds of wheat genotypes were sown in small plastic pots and the drought stress was produced by using different concentrations of PEG and the seed germination, seedling length, moisture content were measured. Based on these, vigour index and stress index were calculated. The results of the study revealed that the MS3 medium was superior to MS1 and MS2 medium for callus induction. The genotypic variation was present for callus induction and the mean callus induction was found the highest in WH-711 (83.85%) and the lowest in PBW-343 (65.66%). Callus regeneration was the highest in genotype PBW-343 (89.58%, from the calli developed on MS3 medium). Similarly, the number of shoots regenerated /callus was higher in WH-533 (9-10, from the calli developed on MS3 medium) and the lowest in WH-147 (5, from the calli developed on MS1 medium). Average seed germination was found the highest in WH-533 and WH-147 (80.00%) but at maximum stress level (17.5% PEG), the germination of PBW-502 (56.67%) was the highest. Moisture content of WH-147 was found the highest (89.44%). When the genotypes were compared for the callus growth percentage and stress index, the genotypes WH-533, WH-147, HD-2687 and C-306 were found better than the other genotypes under study.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/86990
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subPlant Breeding
dc.subjectDrought stress, PEG, Seedling, Wheat, Callus, Seed Germination, Moisture content, Vigour indexen_US
dc.these.typeM.Sc
dc.titleCallus development, plant regeneration and in vitro selection for drought tolerant cell lines of wheat [Triticum aestivum l.]en_US
dc.typeThesisen_US
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