POLYMORPHISM OF CANDIDATE GENES CONTROLLING TEMPERAMENT TRAITS AND THEIR ASSOCIATION ON REPRODUCTION TRAITS IN VECHUR CATTLE

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dc.contributor.advisorManoj M.
dc.contributor.authorANANDAKUMAR PONNALA
dc.date.accessioned2022-11-03T08:01:13Z
dc.date.available2022-11-03T08:01:13Z
dc.date.issued2018-09-30
dc.descriptionSubmitted in partial fulfillment of the requirement for the degree of Master of Veterinary Science of Animal Genetics and Breedingen_US
dc.description.abstractTemperament in cattle plays an important role in animal safety, production and reproduction performance. Bos indicus species are known for their aggressive behaviour than Bos taurus. The present study was conducted to identify the polymorphism of candidate genes controlling temperament traits and their effects on reproductive performance of Vechur cattle. The study comprised of 68 Vechur cattle that were divided into four temperament groups based on their subjective temperament score, social separation test, docile test and handler’s/milker’s score test. Available reproductive performance data of animals were collected from Vechur cattle conservation project to study the following traits - age at first service, age at first calving, service period, conception rate and number of services per conception. Six candidate genes - Tyrosinase (TYR), Dopamine receptor D4 (DRD4), Monoamine Oxidase A (MAO A), Cannabinoid receptor 1 (CNR1), Regulator of G-protein signaling 2 (RGS2) and Plexin A2 (PLXA2) - for temperament were selected. Three selected SNPs from each gene were screened using PCR-RFLP/ PCR-SSCP and further confirmed by sequencing. Out of these 18 SNPs, 9 were present in the Vechur herd. The genes MAOA, TYR, CNR1 and PLXA2 contain two, two, three and two SNPs, respectively. Except TYR 6416322 SNP remaining eight SNPs, coat colour (p=0.003) and sex (p=0.001) were significantly associated with handler’s/milker’s temperament score of handler three (H3). MAOA 105380513 SNP C to T transition was fixed in Vechur population. The average service period of four temperament classes (docile, restless, nervous and aggressive) were 251.02 ± 24.04, 168.57 ± 19.28, 230.59 ± 21.75 and 243.05 ± 32.26 days, respectively. Second restless group had significantly (p=0.039) lower average service period in comparison to all other groups. However, age at first service, age at first calving, conception rate and number of services per conception were not significantly associated with temperament score in the present study. There were significant correlations among different reproductive traits. Results of this study revealed that the selected genes were having significant effect on temperament and reproduction traits, which may be confirmed by further research using larger population and more accurate temperament score. This would enable us to incorporate temperament as an important trait in the selection programmes for genetic improvement of Vechur cattle.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810189558
dc.keywordsPOLYMORPHISM, POLYMORPHISM OF CANDIDATE GENES, TEMPERAMENT TRAITS, REPRODUCTION TRAITS IN VECHUR CATTLE, VECHUR CATTLE, TEMPERAMENT, MEASUREMENT OF TEMPERAMENT,Social separation test (SS), Handling temperament during weighing, Milking temperament, HERITABILITY, Bos taurus vs Bos indicus, GENETIC DIVERSITY, Tyrosinase Gene -TYR, Dopamine receptor D4 gene- DRD4, Plexin A2 gene -PLXNA2, Monoamine Oxidase A -MAO-A, Cannabinoid receptor gene - CNR1, TEMPERAMENT, SINGLE STRAND CONFORMATION POLYMORPHISM (SSCP), SILVER STAINING, POLYMERASE CHAIN REACTION, GROUPING OF VECHUR ANIMALS, Social separation test (SS test), Docile test, Temperament score, Milking / Handling temperament test, COLLECTION OF REPRODUCTIVE DATA, SELECTION OF CANDIDATE GENES, SINGLE 20 NUCLEOTIDE POLYMORPHISMS (SNPS), Extraction of DNA from blood, Agarose gel electrophoresis, Primers used for PCR, Dilution of primers, Setting up of PCR, Agarose gel electrophoresis of PCR product, Allelic and Genotype frequencies, SEQUENCING OF POOLED SAMPLES, SINGLE STRAND CONFORMATION POLYMORPHISM, Silver staining of polyacrylamide gels, Sequencing of polymorphic SSCP pattern, Genotype frequency, Genotype and Allele frequencies, Allele frequency,en_US
dc.language.isoEnglishen_US
dc.pages132 p.en_US
dc.publisherKerala Veterinary and Animal Sciences Mannuthy, Thrissuren_US
dc.research.problemDetection of SNPs in selected candidate genes controlling temperament traits in Vechur cattle , Study the association of these SNPs with reproduction and fertility traits, Study the differential expression of selected candidate genes related to temperament traits by quantitative-PCRen_US
dc.subAnimal Genetics and Breedingen_US
dc.themePOLYMORPHISM OF CANDIDATE GENES CONTROLLING TEMPERAMENT TRAITS AND THEIR ASSOCIATION ON REPRODUCTION TRAITS IN VECHUR CATTLEen_US
dc.these.typeM.V.Sc.en_US
dc.titlePOLYMORPHISM OF CANDIDATE GENES CONTROLLING TEMPERAMENT TRAITS AND THEIR ASSOCIATION ON REPRODUCTION TRAITS IN VECHUR CATTLEen_US
dc.typeThesisen_US
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