Genotoxicity and oxidative stress in earthworms in response to pesticides under natural conditions in submountainous undulating zone of Punjab

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dc.contributor.advisorAulakh, R.K.
dc.contributor.authorJasmeen Kaur
dc.date.accessioned2024-01-03T10:38:29Z
dc.date.available2024-01-03T10:38:29Z
dc.date.issued2022
dc.description.abstractThe expansion of agriculture and extensive use of pesticides disturb soil ecosystem leading to soil pollution, toxicity and significant population damage to soil organisms. The current study was set out to assess the amount of pesticide residue present inside the tissues of earthworms and how oxidative stress and genotoxicity were induced in earthworms due to excess of pesticide residues in agricultural lands of Hoshiarpur and Gurdaspur districts. Pesticide residue analysis revealed the presence of chlorpyrifos and edifenphos in soil whereas no pesticide residues were found above the limit of quantification in earthworm tissues. Genotoxicity test revealed a tail DNA% of 5.61 in case of earthworms taken from chlorpyrifos contaminated soil and a tail DNA% of 21.66 in case of earthworms taken from edifenphos contaminated soil from Hoshiarpur district and a tail DNA% of 0.01 in case of earthworms taken from chlorpyrifos contaminated soil from Gurdaspur district. Cytogenetic analysis showed no chromosomal aberrations were observed. The antioxidative enzyme activity showed a significant difference between the activity of Superoxide Dismutase, Glutathione Peroxidase, Glutathione-S-Transferase and Acetylcholinesterase was observed from earthworms from Hoshiarpur district. A significant difference between the activity of Superoxide Dismutase, Glutathione Peroxidase, Glutathione-S-Transferase was observed from earthworms from Gurdaspur district. It was concluded that there are less effects of pesticide residues on earthworms under field conditions as compared to the previous studies done under controlled lab conditions due to the dynamic and complex nature of the environment which cannot be stimulated in the lab.en_US
dc.identifier.citationJasmeen Kaur (2022) Genotoxicity and oxidative stress in earthworms in response to pesticides under natural conditions in submountainous undulating zone of Punjaben_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810205437
dc.keywordsPesticide residue analysis, Genotoxicity, Cytogenetic study, Comet assay, Karyotyping, earthwormsen_US
dc.language.isoEnglishen_US
dc.pages71en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemGenotoxicity and oxidative stress in earthworms in response to pesticides under natural conditions in submountainous undulating zone of Punjaben_US
dc.subZoologyen_US
dc.themeGenotoxicity and oxidative stress in earthworms in response to pesticides under natural conditions in submountainous undulating zone of Punjaben_US
dc.these.typeM.Scen_US
dc.titleGenotoxicity and oxidative stress in earthworms in response to pesticides under natural conditions in submountainous undulating zone of Punjaben_US
dc.typeThesisen_US
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