Effects of Brassinosteroid on Morphophysiology and Yield of French Bean (Phaseolus vulgaris L.)

Abstract
An experiment entitled “Effects of Brassinosteroid on Morpho-physiology and Yield of French Bean (Phaseolus vulgaris L.)” was conducted during November, 2022 to February, 2023 in the Department of Crop Physiology and in the Experimental Farm, Department of Horticulture, Assam Agricultural University, to evaluate the effects of homobrassinolide on growth and yield of French bean. The experiment was carried out in two phases i.e. A) Laboratory experiment and B) Field experiment. The laboratory experiment was laid out in completely randomized design (CRD) with 4 replications using one variety (Falguni) of the crop. Seeds of French bean were treated with different concentrations of homobrassinolide viz. 0.25 ppm, 0.50 ppm, 0.75 ppm, 1.00 ppm and control (0 ppm) to examine the germination and seedling growth of the tested crop. Among all the applied concentrations of homobrassinolide, 0.50 ppm was found to show superior results in increasing the germination percentage, germination index, shoot and root lengths, shoot and root fresh weights, shoot and root dry weights and vigour index of the tested crop. The field experiment was conducted to investigate the effect of foliar application of homobrassinolide on morpho-physiology and yield development of French bean (variety Falguni). Foliar spray was done at 20 days after sowing (20 DAS) and 40 days after sowing (40 DAS). The experiment was laid out in Randomized block design (RBD) with four (04) replications and nine (09) treatments viz. T1 (0.25 ppm; single application : 20 DAS), T2 (0.50 ppm; single application: 20 DAS), T3 (0.75 ppm; single application: 20 DAS), T4 (1.00 ppm; single application: 20 DAS), T5 (0.25 ppm; double application: 20 DAS + 40 DAS), T6 (0.50 ppm; double application: 20 DAS + 40 DAS), T7 (0.75 ppm; double application: 20 DAS + 40 DAS), T8 (1.00 ppm, double application: 20 DAS + 40 DAS) and T9 (0 ppm; control). Among all the treatments, T2 (0.50 ppm; single application: 20 DAS) exhibited higher increase in plant height, leaf number, shoot and root fresh weights, shoot and root dry weights, chlorophyll content, leaf NPK content, root nodule number, nitrate reductase activity, yield and all yield attributing parameters. Single application of 0.50 ppm homobrassinolide resulted in the highest cost benefit ratio (1: 1.65), where 27.27% increase in yield over control was recorded.
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