COMPARATIVE EVALUATION OF DECELLULARISED BOVINE OMENTUM ALONE AND IN COMBINATION WITH MITOMYCIN-C IN THE MANAGEMENT OF CORNEAL INJURIES IN DOGS
Loading...
Date
2019
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
The study was undertaken to evaluate the efficacy of decellularised bovine omentum alone
and in combination with mitomycin-C for the management of corneal injuries in dogs. All the
cases with corneal injuries which subsequently lead to corneal ulceration were screened and 12
corneas were selected from 11 animals irrespective of breed, age and sex. Out of eleven dogs
selected, 5 were Chinese Pug, 3 were French Bulldog, 2 were Pitbull and one was a Labrador
Retriever. The age of affected animals ranged from 3 months to 38 months with mean value of
13.75 ± 3.09 months and 6 were male and 5 were female. One animal was bilaterally affected and
left and right eyes were affected in equal proportion. Thorough ophthalmic examination was
carried out in all the cases with bilateral evaluation of menace response, palpebral reflex, dazzle
reflex, pupillary light response, Schirmer tear test, Fluorescein dye test, ocular tonometry, direct
and indirect ophthalmoscopy, antibiogram of corneal swabs, corneal impression cytology and slit
lamp biomicroscopy. Twelve corneas selected were randomly divided in to two groups, Group I
and II, consisting of six corneas each for surgical treatment. Under general anaesthesia, corneal
grafting was carried out in Group I whereas Group II corneas underwent single time intra-operative
application of topical mitomycin-C for 2 minutes before corneal grafting with decellularised and
gamma irradiated bovine omentum. All the corneas were protected by temporary tarsorrhaphy
which was retained up to day 7 and observations viz. corneal oedema, neovascularization, extend
of pigmentation, scarring and corneal clarity were serially recorded on day 7, 14, 21 and 60 post
operatively. All the corneas in Group I showed negative FDT by day 7, but in Group II, complete
epithelialization was obtained by day 7 in 3 corneas and rest showed negative FDT by day 14 post
grafting. Corneal oedema subsided by day 14 in all the cases and corneal neovascularization was
at the peak on day 7 and day 14 post-grafting which regressed completely by day 60. Corneal
clarity had improved gradually throughout the observation period. None of the corneas developed
vision impairing corneal melanosis or scarring except one in Group I. Alteration in shape of pupil
occurred as a complication of iridectomy in two cases. From the present study it was concluded
that re-epithelialization of cornea was enhanced by corneal grafting with decellularised bovine
omentum and application of mitomycin-C was effective in controlling corneal fibrosis.