STUDIES ON IMMUNOMODULATORY AND GROWTH PROMOTING EFFECTS OF CINNAMON OIL IN BROILER CHICKEN
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Date
2020
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Department of Veterinary Pharmacology and Toxicology College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand
Abstract
To assess immunomodulatory activity, a total of 60 chicks were divided randomly to 5 groups each of 12 chicks. Group I served as control and was given basal diet without cinnamon oil and vitamin E & selenium. Group II served as standard control and was given vitamin E & selenium containing proprietary product in water at the dose rate of 1.5 grams per 100 birds for first two weeks and 5 grams per 100 birds for next 3 weeks. The remaining groups III, IV and V were given cinnamon oil at the dose rate of 200, 400 and 800 mg/kg diet, respectively. This study was conducted for 35 days. Cutaneous basophil hypersensitivity (CBH) response at two different doses (100 μg and 200 μg) of phytohemagglutinin-P (PHA-P) was carried out to assess the cell mediated immunity on 14th day of age. Blood was collected on 7th, 21st and 35th day of age and serum was separated to estimate antibody titers against ND vaccine by haemagglutination inhibition (HI) test and biochemical parameters like serum total protein, serum albumin, serum globulin and A/G ratio. On 35th day, thin blood smears were prepared and stained with field’s stain to determine differential leucocyte counts microscopically. At the end of the experiment, birds of all groups were slaughtered and tissues like thymus, spleen and bursa were collected for histopathological examinations.