MOLECULAR CHARACTERISATION OF EXTENDED SPECTRUM BETA LACTAMASE (ESBL) PRODUCING Escherichia coli IN POULTRY
| dc.contributor.advisor | Hazarika, A. K. | |
| dc.contributor.author | BASAIAWMOIT, ERNESTINE | |
| dc.date.accessioned | 2018-11-05T09:37:50Z | |
| dc.date.available | 2018-11-05T09:37:50Z | |
| dc.date.issued | 2016-07 | |
| dc.description.abstract | The study was undertaken to isolate and identify Escherichia coli from poultry with or without the history of diarrhoea and to determine the occurrence of extended spectrum beta-lactamase (ESBL) producing Escherichia coli in Assam and Meghalaya, India. A total of 182 (67.40%) samples yielded E. coli which included 106 (67.08%) samples from Assam and 76 (67.85%) samples from Meghalaya. The samples were obtained from cloacal swabs, faecal samples and intestinal contents of diarrhoeic and non-diarrhoeic poultry birds. All the 182 strains of E. coli isolated from diarrhoeic and non-diarrhoeic birds were subjected to antibiotic susceptibility test and were phenotypically confirmed to be ESBL producers by DDST method. A total of 39 (21.42%) samples were confirmed as ESBL producers. Out of these, 19 (17.92%) samples were from Assam and 20 (26.31%) samples were from Meghalaya. Further, the extended-spectrum beta-lactamase genes viz., blaCTX-M, blaTEM, and blaSHV were detected by Polymerase Chain Reaction from the phenotypically confirmed isolates. 17 (9.34%) isolates were found to be positive for at least one of the two resistance genes, viz. blaTEM (686bp) and blaCTX-M (585bp). None of the isolates were found to contain the blaSHV gene. Of the 17 isolates, 5 (2.75%) were found to be positive for blaTEM gene, of which 3 (1.65%) were from Assam and 2 (1.09%) from Meghalaya. Similarly, 12 (6.59%) were found to be positive for blaCTX-M gene, of which 5 (2.75%) were from Assam and 7 (3.85%) were from Meghalaya. Prevalence of the resistant genes in poultry birds was found to be slightly higher in Meghalaya in comparison to Assam. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810082892 | |
| dc.keywords | MOLECULAR CHARACTERISATION, EXTENDED SPECTRUM BETA LACTAMASE (ESBL), Escherichia coli, POULTRY | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 71p. | en_US |
| dc.publisher | Assam Agricultural University, Khanapara, Guwahati | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | MOLECULAR CHARACTERISATION OF EXTENDED SPECTRUM BETA LACTAMASE (ESBL) PRODUCING Escherichia coli IN POULTRY | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | MOLECULAR CHARACTERISATION OF EXTENDED SPECTRUM BETA LACTAMASE (ESBL) PRODUCING Escherichia coli IN POULTRY | en_US |
| dc.type | Thesis | en_US |